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from mouse embryo(blastocyst), 08021401

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biological source

mouse embryo (blastocyst)

growth mode



Not specified


Adherent monolayer of spheroidal cells on feeder layer of mouse primary embryonic fibroblasts or STO cells. The cells spontaneously differentiate and form embryonal structures in the absence of a feeder layer.


Not specified


Not specified


cell culture | mammalian: suitable

shipped in

dry ice

Related Categories

Cell Line Origin

Mouse (strain 129/Ola) embryonic stem cell

Cell Line Description

Pluripotent mouse embryonic stem cell line. ES-E14TG2a cells are deficient in in HPRT and are resistant to 0.06mM 6-thioguanine.

DNA Profile

Not specified

Culture Medium

DMEM + 2 mM Glutamine + 1% NEAA + 0.1 mM 2-Mercaptoethanol + 1000 U/ml mouse inhibitory factor (LIF) + 1.5% sodium bicarbonate + 10% Fetal Bovine Serum. Renewal of medium every 2-3 days is required.

Subculture Routine

Not specified

Other Notes

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