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NIH-3T3 4-2

86041101, mouse skin, Fibroblast

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About This Item

UNSPSC Code:
41106514

product name

NIH-3T3 4-2, 86041101

biological source

mouse skin

growth mode

Adherent

karyotype

Not specified

morphology

Fibroblast

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−196°C

Related Categories

General description

Product has lost contact inhibition

Cell Line Origin

Mouse fibroblast

Cell Line Description

A mouse fibroblast cell line which has an integrated mutant Moloney MuLV. The cell line can be used to generate helper-free defective retroviruses.

Application

Virus studies: Moloney MuLV, retroviruses

Culture Medium

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Certificates of Analysis (COA)

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