V79-HG04

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSC Code: 41106514
• Biological source: hamster lung
• Relevant disease(s): cancer
• Growth mode: Adherent
• Karyotype: Aneuploid
• Morphology: Fibroblast-like

Properties

• biological source: hamster lung
• packaging: tube of 5 μg 87020406-DNA-5UG, pkg of vial of cells 87020406-1VL
• growth mode: Adherent
• karyotype: Aneuploid
• morphology: Fibroblast-like
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Application

Cell fusion studies

Biochem/physiol Actions

A spontaneously transformed cell line of a primary culture derived from embryonic lung fibroblasts of a Chinese hamster. Thioguanine resistant, HAT sensitive, high cloning efficiency in soft agar and used for HPRT- genotyping selection in HAT.

Hamster Chinese embyro lung fibroblast transformed

Preparation Note

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.