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Nthy-ori 3-1 Cell Line human

90011609

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Synonym(s):
HTori-3.1 Cells, Nthy-ori 3.1 Cells

biological source

human thyroid (follicular epithelial)

growth mode

Adherent

karyotype

Not specified

morphology

Epithelial

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−196°C

Related Categories

Cell Line Origin

Human thyroid follicular epithelial

Cell Line Description

Normal human primary thyroid follicular epithelial cells were transfected with a plasmid containing an origin-defective SV40 genome (SV-ori) to immortalise them. SV40 DNA was able to immortalise normal thyroid epithelial cells with the retention of several different functions.

Application

Nthy-ori 3-1 human cell Line has been used for transfection and virus studies. It is also used for the study of control and growth in human thyroid. The cell line has been used for microRNA (miRNA) expression profiling.

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

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