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Key Documents

2-179-E11

from Mouse x Mouse Hybridoma, 92030602, Reactivity Anti-TNF alpha, human )

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About This Item

UNSPSC Code:
41106514

biological source

Mouse x Mouse Hybridoma

ImmunoDon

BALB/c mouse spleen

Myeloma

NS0

Reactivity

Anti-TNF alpha, human

technique(s)

cell culture | mammalian: suitable

isotype

IgG1

shipped in

dry ice

Cell Line Origin

Mouse NS0 x Mouse BALB/c spleen

Cell Line Description

This strongly neutralising antibody is directed against natural and recombinant human TNF alpha. It does not cross-react with TNF ß. It cross-reacts with simian TNF alpha but not with TNF alpha or ß of other species. It can be paired in a sandwich ELISA with 357-101-4. Disclaimer required.

Immunogen

Highly purified recombinant human tumour necrosis factor alpha

Application

Immunoassays, TNF alpha neutralisation, immunohistochemistry

Culture Medium

RPMI 1640 + 2 mM glutamine + 10% Fetal Bovine Serum (FBS) (heat inactivated)

Subculture Routine

Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37 °C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Disclaimer

This cell line has special release conditions: All customers are required to complete a Cell line Release Authorisation form.

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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