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F12

92032412, Reactivity: Anti-Haemolysin, Aeromonas species

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biological source

Mouse x Mouse Hybridoma

ImmunoDon

BALB/c mouse spleen

Myeloma

Sp2/0

Reactivity

Anti-Haemolysin, Aeromonas species

technique(s)

cell culture | mammalian: suitable

isotype

IgG1

shipped in

dry ice

storage temp.

−196°C

Related Categories

Cell Line Origin

Mouse Sp2/0 x Mouse BALB/c spleen

Cell Line Description

The neutralising antibody is directed primarily against the haemolytic activity of Aeromanes sobria but it also neutralises cytotoxic activity. It recognises 3 major polypeptide bands of 52kDa, 47kDa, and 43kDa.

Immunogen

Aeromonas sobria haemidysin, partially purified 60kDa

Culture Medium

RPMI 1640 + 2mM glutamine + 1% Hypoxanthine, Aminopterin, Thymidine (HAT) + 10% Foetal Bovine Serum (FBS)

Subculture Routine

Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.

Other Notes

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