SH-SY5Y Cell Line human
94030304, Neuroblast from neural tissue.
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SHSY5Y Cells, SK-SH-SY5Y Cells, SY5Y Cells
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biological source
human nerve
growth mode
Adherent
karyotype
Not specified
morphology
Neuroblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
metastasis
shipped in
dry ice
storage temp.
−196°C
Related Categories
Cell Line Origin
Human neuroblastoma
Cell Line Description
SH-SY5Y is a thrice-cloned sub-line of bone marrow biopsy-derived line SK-N-SH (Sigma catalogue no. 86012802). SH-SY-5Y has dopamine-β-hydroxylase activity and can convert glutamate to the neurotransmitter GABA. Will form tumours in nude mice in approximately 3-4 weeks. The loss of neuronal characteristics has been described with increasing passage numbers. Therefore it is recommended to verify specific characteristics such as noradrenalin uptake or neuronal markers routinely.
Application
SH-SY5Y cell line human is used for neurotransmitter studies
SH-SY5Y cell line human has been used to:
SH-SY5Y cell line human has been used to:
- study the iron-mediated toxicity of amyloid β peptide
- check the significance of anionic luminescent conjugated polythiophene (LCP) polythiophene acetic acid (PTAA) in vital staining of cells
- study the effect of haloperidol on sigma-1 receptors in guinea pig brain and human neuroblastoma cells
Other Notes
- DNA is stored at -70 °C
- RNA is stored at -70 °C
- Cell line is stored at -196 °C
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DNA Profile
STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 11
D16S539: 8,13
D5S818: 12
D7S820: 7,10
THO1: 7,10
TPOX: 8,11
vWA: 14,18
CSF1PO: 11
D13S317: 11
D16S539: 8,13
D5S818: 12
D7S820: 7,10
THO1: 7,10
TPOX: 8,11
vWA: 14,18
Culture Medium
Ham′s F12:EMEM (EBSS) (1:1) + 2mM Glutamine + 1% Non Essential Amino AcidsNEAA + 15% Foetal Bovine Serum FBS / FCS.
Subculture Routine
Split sub-confluent cultures (70-80%) 1:10 to 1:100 i.e. seeding at 1x1,000-1x10,000 cells/cm2; using 0.25% Trypsin or Trypsin/EDTA, 5% CO2; 37°C. Cells may reattach slowly and may remain in suspension for several days. The loss of neuronal characteristics has been described with increasing passage numbers. Therefore it is recommended to verify specific characteristics such as noradrenalin uptake or neuronal markers routinely.
Certificates of Analysis (COA)
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