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DMS 273

95062830

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Synonym(s):
DMS273 Cells

biological source

human lung (effusion)

growth mode

Adherent

karyotype

Not specified

morphology

Not specified

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Related Categories

Cell Line Origin

Human lung, small cell carcinoma

Cell Line Description

DMS 273 was initiated in 1978 from the pleural fluid specimen from a 50 year old female patient with small cell carcinoma of the lung. The patient was in relapse after chemotherapy and radiotherapy. DMS 273 cells exhibit intercellular processes and express retinoblastoma mRNA and protein. The cell line is tumorigenic in nude mice.

Application

Human lung tumour model

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 10 ,12
D13S317: 12,13
D16S539: 11
D5S818: 11,12
D7S820: 10,12
THO1: 9.3
TPOX: 10
vWA: 15

Culture Medium

Waymouth′s MB 752/1 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) (Heat Inactivated).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:4 i.e. seeding at 4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

Other Notes

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