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biological source

mouse embryo

growth mode



Not specified




Not specified


Not specified


cell culture | mammalian: suitable

shipped in

dry ice

storage temp.


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Cell Line Origin

Mouse embryo fibroblast, SV40 - transformed

Cell Line Description

The cell line MEF-1 was established by transfection of mouse embryo fibroblasts ((C57 BL/6 x 129) F1) with a SV40 coding plasmid. MEF-1 express the wild-type for low density lipoprotein receptor related protein (LRP). Together with the cell lines PEA10 (Sigma Catalogue number. 97040310) and PEA13 (Sigma Catalogue number. 97040311), which express heterozygous and homozygous forms of LPR, an experimental system is available for the analysis of cellular uptake of functionally diverse ligands and the effect of LPR - deficiency. This is of importance since LRP - deficient mouse embryos die early during gestation.


Study of LRP and LRP-deficiency

Culture Medium

DMEM + 2 mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:6 to 1:10 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C

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