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CompoZr® Targeted Integration Kit - mRosa26
Transgene Integration in the Mouse Genome
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About This Item
UNSPSC Code:
12352200
NACRES:
NA.51
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General description
The CompoZr Targeted Integration Kit - mRosa26 is designed to rapidly integrate a user-specified gene of interest (GOI) into the preferred site of integration in the mouse genome, the Rosa 26 locus. The well characterized Zinc Finger Nucleases (ZFNs) in this kit target the Rosa26 locus with high specificity, creating a double strand break. ZFN treated cells then use a pZDonor plasmid with homology arms to the Rosa26 genomic locus to repair the ZFN created double stranded break by the natural repair process of homology directed repair. The pZDonor plasmid can be engineered to contain your GOI and will direct targeted integration of your GOI into the Rosa26 locus. <http://www.sigmaaldrich.com/life-science/zinc-finger-nuclease-technology/compozr-kit.html>The CompoZr Targeted Integration Kit - mRosa26 provides a common integration site within a defined locus to generate stable, uniform transgene expression levels.
Application
CompoZr® targeted integration kit - mRosa26 has been used to target the Rosa26 locus in mouse zygotes.
Features and Benefits
Effective Integration Efficiency
No Transgene Landing Pads Required
Rapid Biallelic Integrations with a Single Transfection
* This kit was developed for targeted integration applications using cell lines and has not been optimized for use with embryo microinjection methods used to make genetically modified animals. For detailed information on potential applications with this kit please contact Sigma Technical Service.
- Any mouse cell type can be modified with the CompoZr Targeted Integration Kit - mRosa26
- The ZFNs are a well-validated pair that cleaves the target DNA with high specificity
- ZFN mediated targeted integration can be achieved with high success rates
No Transgene Landing Pads Required
- Prior to ZFN transfection cell lines don′t require engineering of transgene landing pads
- Lack of need for transgene landing pads enables immediate transfection of cell lines with GOI
Rapid Biallelic Integrations with a Single Transfection
- Monoallelic and biallelic integrations have been generated with ZFNs
- Gene integrations can occur in as little as 48 hours
* This kit was developed for targeted integration applications using cell lines and has not been optimized for use with embryo microinjection methods used to make genetically modified animals. For detailed information on potential applications with this kit please contact Sigma Technical Service.
Legal Information
All Zinc Finger Nucleases are sold under license from Sangamo Biosciences. For a copy of the Label License provided with purchase of CompoZr ZFNs, please visit the ZFN Label License page.
CompoZr is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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Efficient gene targeting of the Rosa26 locus in mouse zygotes using TALE nucleases
Petr Kasparek
Febs Letters (2014)
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