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CWA-1012

Sigma-Aldrich

Rat IgG2b Isotype Control (LTF-2) ColorWheel® Dye-Ready mAb

greener alternative

for use with ColorWheel® Dyes (Required, sold separately)

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biological source

rat

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

LTF-2, monoclonal

product line

ColorWheel®

form

lyophilized

species reactivity

rat

packaging

antibody small pack of 25 μL

greener alternative product characteristics

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

technique(s)

flow cytometry: suitable

isotype

IgG2b

compatibility

for use with ColorWheel® Dyes (Required, sold separately)

greener alternative category

shipped in

ambient

storage temp.

2-8°C

target post-translational modification

unmodified

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purified antibody

clone

LTF-2, monoclonal

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C1.18.4, monoclonal

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11E10, monoclonal

product line

ColorWheel®

product line

ColorWheel®

product line

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product line

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form

lyophilized

form

lyophilized

form

lyophilized

form

lyophilized

species reactivity

rat

species reactivity

rat

species reactivity

mouse

species reactivity

-

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General description

ColorWheel® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any ColorWheel® antibody with any ColorWheel® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each ColorWheel® antibody and dye is lyophilized for long long-term storage, allowing for simplicity and flexibility without compromise. ColorWheel® technology requires both ColorWheel® antibody and ColorWheel® dye for Flow Cytometry application.
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This product is Preservative-free, lyophilized product for enhanced stability and allow for ambient shipping and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

Specificity

Clone LTF-2 is an isotype control antibody for rat IgG2b.

Application

Quality Control Testing:

Evaluated by Flow Cytometry in Protein A/G beads.

Rat IgG2b Isotype Control (LTF-2) ColorWheel® Dye-Ready mAb was tested by flow cytometry using Protein A/G beads.

Compatibility

Requires ColorWheel® Dye (Sold Separately)

Target description

Isotype control antibodies are ideal negative controls for various immunological applications. They can help discriminate between specific antibody signal from non-specific background signal. In selecting an isotype control consideration of host, isotype, conjugation, light chain, and concentration are important. It should be of the same species and isotype as the antibody of interest and must be used at the same concentration and conjugated to the same fluorophore. Rat IgG2b Isotype Control, clone LTF-2 is suitable for use as an isotype-matched control for rat IgG2b. It has an unknown binding specificity and can be used for evaluation of background staining caused by non-specific interactions between a rat IgG2b and the sample under defined experimental conditions.

Physical form

Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted antibody solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.

Reconstitution

1.0 mg/mL after reconstitution at 25 μL in PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 μL of PBS. Protect from light.

Legal Information

ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

related product

Product No.
Description
Pricing

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Articles

Find answers to frequently asked questions (FAQs) about ColorWheel® flow cytometry antibodies and dyes, straight from the experts.

Harness the flexibility of ColorWheel® flow cytometry antibodies and dyes to unlock freedom in your flow cytometry multiplex assay design or multicolor panel design and simplify your multiplex flow cytometry workflows.

Protocols

Experience simplicity in your flow cytometry workflow with the 3-Step ColorWheel® Flow Cytometry Reagent Preparation protocol. With less than 5 minutes of hands-on time, see how simple it is to create your own optimal flow cytometry reagents with ColorWheel® flow cytometry antibodies and dyes.

View ColorWheel® protocol steps for flow cytometry analysis when using ColorWheel® antibodies with ColorWheel® dyes including antibody preparation, PBMC sample preparation, cell surface staining, and intracellular (cytoplasmic) staining.

Related Content

Unlock freedom in your flow cytometry assay design with ColorWheel® flow cytometry antibodies and dyes. The ColorWheel® flow cytometry portfolio was designed with simplicity and flexibility in mind to help streamline your flow cytometry workflow without compromising on quality.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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