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Anti-Human CD63 (MEM-259) ColorWheel® Dye-Ready mAb

greener alternative

for use with ColorWheel® Dyes (Required, sold separately)

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biological source


Quality Level

antibody form

purified antibody

antibody product type

primary antibodies


MEM-259, monoclonal

product line




mol wt

calculated mol wt 25.64 kDa

species reactivity



antibody small pack of 25 μL

greener alternative product characteristics

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.


flow cytometry: suitable



epitope sequence


Protein ID accession no.

UniProt accession no.


for use with ColorWheel® Dyes (Required, sold separately)

greener alternative category

shipped in


storage temp.


target post-translational modification


Gene Information

human ... CD63(967)

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Gene Information

human ... CD63(967)

Gene Information

mouse ... IL2RA(3559)

Gene Information

human ... ITGB1(3688)

Gene Information

mouse ... FCGR1A(2209)


MEM-259, monoclonal


BC96, monoclonal


MEM-101A, monoclonal


10.1, monoclonal

species reactivity


species reactivity


species reactivity


species reactivity


antibody form

purified antibody

antibody form

purified antibody

antibody form

purified antibody

antibody form

purified antibody

product line


product line


product line


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General description

ColorWheel® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any ColorWheel® antibody with any ColorWheel® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each ColorWheel® antibody and dye is lyophilized for long long-term storage, allowing for simplicity and flexibility without compromise. ColorWheel® technology requires both ColorWheel® antibody and ColorWheel® dye for Flow Cytometry application.
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This product is Preservative-free, lyophilized product for enhanced stability and allow for ambient shipping and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.


Clone MEM-259 is a mouse monoclonal antibody that detects human CD63.


HPB-Acute lymphocytic leukemia cells.


Quality Control Testing:

Evaluated by Flow Cytometry in K562 cells.

Flow Cytometry Analysis: Staining of one million human K562 cells was performed using 5 μL of a 1:1 mixture of Cat. No. CWA-1034, Anti-Human CD63 (MEM-259) ColorWheel® Dye-Ready mAb and Cat. No. CWD-PE ColorWheel® activated Phycoerythrin (PE) Dye or an equivalent amount of PE-conjugated Mouse IgG1 Isotype Control.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user


Requires ColorWheel® Dye (Sold Separately)

Target description

CD63 antigen (UniProt: P08962; also known as Granulophysin, Lysosomal-associated membrane protein 3, LAMP-3, Melanoma-associated antigen ME491, OMA81H, Ocular melanoma-associated antigen, Tetraspanin-30, Tspan-30, CD63) is encoded by the CD63 (also known as MLA1, TSPAN30) gene (Gene ID: 967) in human. CD63 is a multi-pass membrane protein of the tetraspan family that is found on endosome, lysosome, and plasma membranes. CD63 has been detected in platelets, dysplastic nevi benign moles, radial growth phase primary melanomas, hematopoietic cells, and in tissue macrophages. In melanoma cells, it is involved in their motility and adhesion. CD63 also plays a role in the adhesion of leukocytes onto endothelial cells. It is reported to play a role in the activation of ITGB1 and integrin signaling, leading to the activation of AKT, FAK/PTK2 and MAP kinases and promote cell survival, reorganization of the actin cytoskeleton, cell adhesion, spreading and migration. CD63 is a highly N-glycosylated protein with three asparagine glycosylation sites (aa 130, 150, 172) and its ribophorin II (RPN2)-mediated glycosylation has been linked to breast cancer. Overexpression of CD63 has been observed in esophageal cancer that is negatively correlated with tumor stage and lymph node metastasis. Lack of expression of CD63 in platelets has been observed in a patient with Hermansky-Pudlak syndrome (HPS), an autosomal recessive disorder that is characterized by oculocutaneous albinism, bleeding due to platelet storage pool deficiency, and lysosomal storage defects. (Ref.: Lai, X., et al. (2017). Oncol. Let. 13(6); 4245-4251; Tominaga, N., et al. (2014). Mol. Cancer 13; 134; Smith, M., et al. (1997). Melanoma Res. 7 (Suppl. 2), 163-170).

Physical form

Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted antibody solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.


1.0 mg/mL after reconstitution at 25 μL in PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 μL of PBS. Protect from light.

Legal Information

ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Related product

Product No.

Storage Class

11 - Combustible Solids




Not applicable


Not applicable

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Harness the flexibility of ColorWheel® flow cytometry antibodies and dyes to unlock freedom in your flow cytometry multiplex assay design or multicolor panel design and simplify your multiplex flow cytometry workflows.


Experience simplicity in your flow cytometry workflow with the 3-Step ColorWheel® Flow Cytometry Reagent Preparation protocol. With less than 5 minutes of hands-on time, see how simple it is to create your own optimal flow cytometry reagents with ColorWheel® flow cytometry antibodies and dyes.

View ColorWheel® protocol steps for flow cytometry analysis when using ColorWheel® antibodies with ColorWheel® dyes including antibody preparation, PBMC sample preparation, cell surface staining, and intracellular (cytoplasmic) staining.

Related Content

Unlock freedom in your flow cytometry assay design with ColorWheel® flow cytometry antibodies and dyes. The ColorWheel® flow cytometry portfolio was designed with simplicity and flexibility in mind to help streamline your flow cytometry workflow without compromising on quality.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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