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CWA-1087

Sigma-Aldrich

Anti-Mouse IFN-γ (XMG1.2) ColorWheel® Dye-Ready mAb

greener alternative

for use with ColorWheel® Dyes (Required, (Sold Separately))

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Synonym(s):
Interferon gamma

biological source

rat

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

XMG1.2, monoclonal

product line

ColorWheel®

form

lyophilized

mol wt

calculated mol wt 17.91 kDa

purified by

using Protein A

species reactivity

mouse

packaging

antibody small pack of 25 μL

greener alternative product characteristics

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

technique(s)

flow cytometry: suitable

isotype

IgG1κ

epitope sequence

Unknown

Protein ID accession no.

UniProt accession no.

compatibility

for use with ColorWheel® Dyes (Required, (Sold Separately))

greener alternative category

shipped in

ambient

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

mouse ... Ifng(15978)

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This Item
CWA-1085CWA-1137CWA-1151
biological source

rat

biological source

rat

biological source

hamster

biological source

rat

clone

XMG1.2, monoclonal

clone

93, monoclonal

clone

MAR-1, monoclonal

clone

53-6.7, monoclonal

antibody form

purified antibody

antibody form

purified antibody

antibody form

purified antibody

antibody form

purified antibody

technique(s)

flow cytometry: suitable

technique(s)

flow cytometry: suitable

technique(s)

flow cytometry: suitable

technique(s)

flow cytometry: suitable

form

lyophilized

form

lyophilized

form

lyophilized

form

lyophilized

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General description

ColorWheel® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any ColorWheel® antibody with any ColorWheel® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each ColorWheel® antibody and dye is lyophilized for long long-term storage, allowing for simplicity and flexibility without compromise. ColorWheel® technology requires both ColorWheel® antibody and ColorWheel® dye for Flow Cytometry application.
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This product is Preservative-free, lyophilized product for enhanced stability and allow for ambient shipping and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

Specificity

Clone XMG1.2 is a rat monoclonal antibody that detects murine IFN- .

Immunogen

Recombinant murine Interferon- expressed in E.coli.

Application

Quality Control Testing

Evaluated by Flow Cytometry in PMA/Ionomycin-stimulated mouse splenocytes.

Flow Cytometry Analysis: One million BALC/c mouse splenocytes were unstimulated or stimulated with 50 ng/mL of Phorbol 12-myristate 13-acetate (PMA) and 1 μg/mL of ionomycin for 6 hours, and 3 μg/mL Brefeldin A and 2 μM of monensin were added 3 hours before harvesting cells. Cells were stained with APC Anti-Mouse CD3e, fixed, permeabilized and subsequently stained with 5 μL of a 1:1 mixture of Cat. No. CWA-1087, Anti-Mouse IFN- (XMG1.2) ColorWheel® Dye-Ready mAb and Cat. No. CWDS-PE ColorWheel® Antibody-Ready Phycoerythrin (PE) Dye.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Compatibility

Requires ColorWheel® Dye (Sold Separately)

Target description

Interferon - (UniProt: P01580; also known as IFN- ) is encoded by the Ifng gene (Gene ID: 15978) in murine species. IFN- is a pleiotropic molecule with antiproliferative, pro-apoptotic, and antitumor properties. It is a type II homodimeric interferon that is synthesized with a signal peptide (aa 1-22), which is subsequently cleaved off to produce the mature form. It is secreted predominantly by activated lymphocytes such as CD4 T helper type 1, CD8 cytotoxic T cells, T cells, and natural killer cells, and antigen-presenting cells and plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation. Its expression is induced by mitogens and cytokines. IFN- signaling coordinates several biological responses involved in host defense and immune surveillance, establishment of adaptive immunity, and the regulation of inflammation. IFN- is reported to activate macrophages toward a pro-inflammatory profile increasing their phagocytic ability and enhancing their microbial killing activity. It is also shown to upregulate cell surface MHC class II on antigen presenting cells, thus promoting peptide-specific activation of CD4+ T cells. Its diverse biological activities are initiated by IFN- -mediated aggregation of at least two different cell surface receptors: IFN- R1 and IFN- R2. It primarily signals through the JAK-STAT pathway after interaction with IFN- R1 to affect gene regulation. Upon IFN- binding, intracellular domain of IFN- R1 opens out to allow association of downstream signaling components JAK2, JAK1 and STAT1, leading to STAT1 activation, nuclear translocation and transcription of IFN- -regulated genes. Mutations in Ifng gene are known to cause aplastic anemia that is characterized by failure of bone marrow to produce adequate number of peripheral blood elements leading to peripheral pancytopenia and hypoplasia.

Physical form

Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted antibody solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.

Reconstitution

1.0 mg/mL after reconstitution at 25 μL in PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 μL PBS. Protect from light.

Legal Information

ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

required but not provided

Product No.
Description
Pricing

Storage Class

11 - Combustible Solids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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Articles

Harness the flexibility of ColorWheel® flow cytometry antibodies and dyes to unlock freedom in your flow cytometry multiplex assay design or multicolor panel design and simplify your multiplex flow cytometry workflows.

Protocols

Simplify flow cytometry with ColorWheel® Reagent Preparation for optimal antibodies and dyes in under 5 minutes.

ColorWheel® protocol guides flow cytometry analysis with ColorWheel® antibodies and dyes for precise results.

Related Content

Unlock freedom in your flow cytometry assay design with ColorWheel® flow cytometry antibodies and dyes. The ColorWheel® flow cytometry portfolio was designed with simplicity and flexibility in mind to help streamline your flow cytometry workflow without compromising on quality.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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