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CWDS-AF488

Sigma-Aldrich

ColorWheel® Antibody-Ready Alpha Fluor 488 Dye

greener alternative

for use with ColorWheel® Dye-ready Antibodies (Required, sold separately)

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product line

ColorWheel®

Quality Level

form

lyophilized

greener alternative product characteristics

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

technique(s)

flow cytometry: suitable

fluorescence

λex 499 nm; λem 520 nm

compatibility

for use with ColorWheel® Dye-ready Antibodies (Required, sold separately)

greener alternative category

shipped in

ambient

storage temp.

2-8°C

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CWDS-IF405CWDS-IF560CWDS-MF780
fluorescence

λex 499 nm; λem 520 nm

fluorescence

λex 403 nm; λem 427 nm

fluorescence

λex 560 nm; λem 571 nm

fluorescence

λex 629 nm; λem 767 nm

form

lyophilized

form

lyophilized

form

lyophilized

form

lyophilized

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

General description

ColorWheel® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any ColorWheel® antibody with any ColorWheel® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each ColorWheel® antibody and dye is lyophilized for long long-term storage, allowing for simplicity and flexibility without compromise. ColorWheel® technology requires both ColorWheel® antibody and ColorWheel® dye for Flow Cytometry application.

Alpha Fluor 488 NHS Ester (Succinimidyl Ester) is a water soluble, bright green fluorescent dye that exhibits an excitation max at 499 nm and an emission max at 520 nm with an extinction coefficient of 73,000 cm-1 M-1. It is suitable for use over a wide range of pH (4 to 10). It can be used with the 488 nm Argon laser and its spectral properties are identical to Alexa Fluor 488. The NHS ester is the most convenient amine-reactive form for conjugating it to proteins and antibodies and can be used for stable signal generation in Flow cytometry. It displays high photostability and brighter fluorescence.
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This product is Preservative-free, lyophilized product for enhanced stability and allow for ambient shipping and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

application

Flow Cytometry: Excitation: 499 nm, Emission: 520 nm

Compatibility

Requires ColorWheel® Antibody (Sold Separately)

Physical form

CWDS-AF488-25UL Set Components

CWD-AF488-25UL - ColorWheel® Antibody-Ready Alpha Fluor 488 Dye
Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.

CWD-FINISH-25UL -ColorWheel® Finishing Buffer
Lyophilized from PBS. Contains no biocide or preservatives, such as azide.

Reconstitution

Reconstitute each vial by adding 25 μL PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 μL of PBS. Protect from light.

Legal Information

ALEXA FLUOR is a trademark of Life Technologies
ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3


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Articles

Find answers to frequently asked questions (FAQs) about ColorWheel® flow cytometry antibodies and dyes, straight from the experts.

Harness the flexibility of ColorWheel® flow cytometry antibodies and dyes to unlock freedom in your flow cytometry multiplex assay design or multicolor panel design and simplify your multiplex flow cytometry workflows.

Protocols

Simplify flow cytometry with ColorWheel® Reagent Preparation for optimal antibodies and dyes in under 5 minutes.

ColorWheel® protocol guides flow cytometry analysis with ColorWheel® antibodies and dyes for precise results.

Related Content

Unlock freedom in your flow cytometry assay design with ColorWheel® flow cytometry antibodies and dyes. The ColorWheel® flow cytometry portfolio was designed with simplicity and flexibility in mind to help streamline your flow cytometry workflow without compromising on quality.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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