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CWDS-PERCP

Sigma-Aldrich

ColorWheel® Antibody-Ready PerCP Dye

greener alternative

for use with ColorWheel® Dye-ready Antibodies (Required, sold separately)

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product line

ColorWheel®

Quality Level

form

lyophilized

greener alternative product characteristics

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

technique(s)

flow cytometry: suitable

fluorescence

λex 477 nm; λem 678 nm

compatibility

for use with ColorWheel® Dye-ready Antibodies (Required, sold separately)

greener alternative category

shipped in

ambient

storage temp.

2-8°C

General description

ColorWheel® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any ColorWheel® antibody with any ColorWheel® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each ColorWheel® antibody and dye is lyophilized for long long-term storage, allowing for simplicity and flexibility without compromise. ColorWheel® technology requires both ColorWheel® antibody and ColorWheel® dye for Flow Cytometry application.

PerCP (Peridinin-chlorophyll-protein complex) is a water-soluble dye that is isolated from a Dinophyceae sp., a photosynthetic dinoflagellate. It exhibits an excitation max at 477 nm and an emission max at 678 nm and has an extinction coefficient of 350,000 cm-1 M-1. PerCP is commonly used for fluorescent immunolabeling, particularly in applications involving fluorescent-activated cell sorting (FACS) and can be used for multicolor analysis with FITC, phycoerythrin, and other fluorochromes. Due to its photobleaching characteristics, PerCP conjugates may not be used on flow cytometers with high-power lasers.
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This product is Preservative-free, lyophilized product for enhanced stability and allow for ambient shipping and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

Application

Quality Control Testing:

Flow Cytometry: Excitation: 477 nm, Emission: 678 nm

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Compatibility

Requires ColorWheel® Antibody (Sold Separately)

Physical form

CWDS-PERCP-25UL Set ComponentsCWD-PERCP-25UL - ColorWheel® Antibody-Ready PerCP DyeLyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.CWD-FINISH-25UL - ColorWheel® Finishing BufferLyophilized from PBS. Contains no biocide or preservatives, such as azide.

Reconstitution

Reconstitute each vial by adding 25 μL PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 μL of PBS. Protect from light.

Legal Information

ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3


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Articles

Find answers to frequently asked questions (FAQs) about ColorWheel® flow cytometry antibodies and dyes, straight from the experts.

Harness the flexibility of ColorWheel® flow cytometry antibodies and dyes to unlock freedom in your flow cytometry multiplex assay design or multicolor panel design and simplify your multiplex flow cytometry workflows.

Protocols

ColorWheel® protocol guides flow cytometry analysis with ColorWheel® antibodies and dyes for precise results.

Related Content

Unlock freedom in your flow cytometry assay design with ColorWheel® flow cytometry antibodies and dyes. The ColorWheel® flow cytometry portfolio was designed with simplicity and flexibility in mind to help streamline your flow cytometry workflow without compromising on quality.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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