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D0405

Sigma-Aldrich

Dialysis tubing, high retention seamless cellulose tubing

avg. flat width 23 mm (0.9 in.), MWCO 14000, 99.99% retention

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NACRES:
NB.22

avg. diam.

15 mm , when full

avg. flat width

23 mm (0.9 in.)

capacity

~50 mL/ft

pore size

14000 MWCO, 99.99% retention

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General description

Retains >99% of cytochrome c (M.W. 12,400) in solution over a 10-hour period.

Packaging

Supplied in rolls, dry

Quality

May contain glycerin and sulfur compounds in more than trace amounts; washing instructions are included.

Preparation Note

Removal of glycerol included as a humectant can be accomplished by washing the tubing in running water for 3-4 hours. Removal of sulfur compounds can be accomplished by treating the tubing with a 0.3% (w/v) solution of sodium sulfide at 80°C for 1 minute. Wash with hot water (60°C) for 2 minutes, followed by acidification with a 0.2% (v/v) solution of sulfuric acid, then rinse with hot water to remove the acid. This tubing will retain most proteins of molecular weight 12,000 or greater.

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