D2376
Deoxyribonucleic acid methyl green
DNAse substrate
Synonym(s):
Deoxyribonucleic acid–methyl green complex, DNA-Methyl Green
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General description
Methyl green is a widely used nuclei counterstain for histochemical methods. Methyl green binds to the DNA major groove and is used for tracking triplex formation. The positive charge on the aniline rings in the methyl green enables its strong binding to DNA. Methyl green aids clear visualization in confocal laser scanning microscopy.
Application
Deoxyribonucleic acid methyl green has been used in DNA methyl green bioassay.
Substrates
Substrate for DNase
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Certificates of Analysis (COA)
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Simultaneous detection of DAB and methyl green signals on apoptotic nuclei by confocal laser scanning microscopy
Acta Histochemica Et Cytochemica, 36(4), 367-376 (2003)
Synthesis, DNA-binding, and Antiviral Activity of Certain Pyrazolo [3, 4-d] pyrimidine Derivatives
Arch. Pharm. (Weinheim), 333(4), 99-103 (2000)
Methyl green: A DNA major-groove binding drug
Febs Letters, 315(1), 61-64 (1993)
Cytotoxic and DNA interaction activities of extracts from medicinal plants used in Argentina
Journal of Ethnopharmacology, 71(1-2), 145-151 (2000)
Application of the DNA-Specific Stain Methyl Green in the Fluorescent Labeling of Embryos
Journal of Visualized Experiments, 315(99) (2015)
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