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E8897

Sigma-Aldrich

Monoclonal Anti-Ezrin antibody produced in mouse

clone 3C12, ascites fluid

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Synonym(s):
Anti-CVIL, Anti-CVL, Anti-HEL-S-105, Anti-VIL2
MDL number:
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

3C12, monoclonal

mol wt

antigen 80 kDa

contains

15 mM sodium azide

species reactivity

human, mouse, hamster, monkey, kangaroo rat, bovine

technique(s)

immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunoprecipitation (IP): suitable
microarray: suitable
western blot: 1:4,000 using cultured human fibroblast cell line extract

isotype

IgG1

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... EZR(7430)
mouse ... Ezr(22350)
rat ... Ezr(54319)

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This Item
E1281SAB4200806SAB4503077
species reactivity

human, mouse, hamster, monkey, kangaroo rat, bovine

species reactivity

human, rat

species reactivity

bovine, canine, hamster, kangaroo, human, monkey, rat, mouse

species reactivity

mouse, rat, human

antibody form

ascites fluid

antibody form

affinity isolated antibody

antibody form

purified from hybridoma cell culture

antibody form

affinity isolated antibody

biological source

mouse

biological source

rabbit

biological source

mouse

biological source

rabbit

conjugate

unconjugated

conjugate

unconjugated

conjugate

-

conjugate

unconjugated

Gene Information

human ... EZR(7430)
mouse ... Ezr(22350)
rat ... Ezr(54319)

Gene Information

human ... EZR(7430)
mouse ... Ezr(22350)
rat ... Ezr(54319)

Gene Information

mouse ... Ezr(22350)

Gene Information

human ... EZR(7430)

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General description

Ezrin is a member of the best studied member of the ERM family of proteins. The ERM family regulates the dynamic cell membrane functions such as endocytosis, exocytosis, and transmembrane and other cortical signalling pathways. Ezrin (molecular weight 80 kDa) interacts with transmembrane proteins and F-actin and mediate important signalling pathways including RhoA and Hedgehog signalling. Ezrin is also present in the microvillus surface of acid-secreting parietal cells of the gastric glands, where it presents as a family of isoelectric variants. By immunoelectron microscopy of human choriocarcinoma cells, ezrin is enriched just inside the plasma membrane of microvilli and less abundant on more planar aspects of the membrane.
Ezrin is a member of the best studied member of the ezrin/radixin/moesin (ERM) family of proteins. It is present in the microvillus surface of acid-secreting parietal cells of the gastric glands, where it presents as a family of isoelectric variants. By immunoelectron microscopy of human choriocarcinoma cells, ezrin is enriched just inside the plasma membrane of microvilli and less abundant on more planar aspects of the membrane.
Monoclonal Anti-Ezrin (mouse IgG1 isotype) is derived from the 3C12 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Ezrin is present in microvilli, placental microvilli, microspikes, and membrane ruffles of cultured cells. It is also found in the growth cones of cultured neurons and the marginal band of avian erythrocytes. Ezrin comprises a (4.1 protein, ezrin, radixin and moesin) FERM domain, an α-helical linker region and a C-terminal domain. The amino-terminal domain of ezrin is localized to membranes and the carboxy-terminal part, containing the α-helical domain, colocalizes with actin filaments. Ezrin gene is mapped to the human chromosome location 6q25.3.

Specificity

Monoclonal Anti-Ezrin recognizes ezrin (80 kDa). Cross-reactivity is observed with human, monkey, bovine, rat, kangaroo, hamster, and mouse

Immunogen

carboxy-terminal section of recombinant human ezrin (amino acids 362-585).

Application

The antibody was used for detection of Ezrin by immunostaining, immunoblotting in human bladder cancer cells , immunohistochemistry, immunoprecipitation in kidney and colon adenocarcinoma cells and electron microscopy in astrocytes

Biochem/physiol Actions

Ezrin links the cytoskeleton to the plasma membrane, based on its amino acid sequence homologies with other cytoskeletal proteins. It regulates cell growth, migration, and cell adhesion. Ezrin is also implicated in cancer cell invasion and metastasis. The proteins of the ezrin family serve as tyrosine kinase phosphorylation substrates. They exhibit a growth factor receptor-specific pattern of phosphorylation. Ezrin is phosphorylated by epidermal growth factor receptor and platelet-derived growth factor receptor.
The ezrin/radixin/moesin (ERM) family regulates the dynamic cell membrane functions such as endocytosis, exocytosis, and transmembrane and other cortical signalling pathways. Ezrin (molecular weight 80 kDa) interacts with transmembrane proteins and F-actin and mediate important signalling pathways including RhoA and Hedgehog signalling.

Physical form

Monoclonal Anti-Ezrin is provided as ascites fluid with 15 mM sodium azide as a preservative.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

13 - Non Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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A Derouiche et al.
Glia, 36(3), 330-341 (2001-12-18)
Astrocytes extend thin lamellate processes in the neuropil, in particular around synapses, where they can modulate synaptic function or mediate glial-neuronal communication. Previous studies have shown that these lamellate perisynaptic processes change their shape in response to neuronal activity, but
Monique Arpin et al.
Cell adhesion & migration, 5(2), 199-206 (2011-02-24)
The highly related ERM (Ezrin, Radixin, Moesin) proteins provide a regulated linkage between the membrane and the underlying actin cytoskeleton. They also provide a platform for the transmission of signals in responses to extracellular cues. Studies in different model organisms
Subcellular distribution and expression of cofilin and ezrin in human colon adenocarcinoma cell lines with different metastatic potential
Nowak D et al
European Journal of Histochemistry, 52, e14-e14 (2010)
A Bretscher et al.
Journal of cell science, 110 ( Pt 24), 3011-3018 (1998-02-21)
The cortical cytoskeleton of eucaryotic cells provides structural support to the plasma membrane and also contributes to dynamic processes such as endocytosis, exocytosis, and transmembrane signaling pathways. The ERM (ezrin-radixin-moesin) family of proteins, of which ezrin is the best studied
Haydar Çelik et al.
Molecular and cellular biology, 35(18), 3145-3162 (2015-07-08)
Ezrin is a key regulator of cancer metastasis that links the extracellular matrix to the actin cytoskeleton and regulates cell morphology and motility. We discovered a small-molecule inhibitor, NSC305787, that directly binds to ezrin and inhibits its function. In this

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