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F0628

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Ferredoxin-NADP+ Reductase from Spinacia oleracea (spinach)

lyophilized powder, ≥15 units/mg solid, secondary activity: ≥10 units/mg solid NADPH diaphorase

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Synonym(s):
Ferridoxin-NADP+ oxidoreductase
CAS Number:
Enzyme Commission number:
MDL number:
NACRES:
NA.54

form

lyophilized powder

Quality Level

specific activity

≥15 units/mg solid

foreign activity

NADH diaphorase activity ≤10 units/mg solid

storage temp.

−20°C

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F3013P9752F5875
vibrant-m

P9752

Phosphoriboisomerase from spinach

form

lyophilized powder

form

lyophilized powder

form

partially purified powder

form

buffered aqueous solution

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−70°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

foreign activity

NADH diaphorase activity ≤10 units/mg solid

foreign activity

-

foreign activity

-

foreign activity

-

General description

Ferredoxin-NADP+ Reductase is a ferredoxin-soluble partner with a molar ratio to PSI of 0.9 and 3.

Application

Ferredoxin-NADP+ Reductase from Spinacia oleracea (spinach) has been used to test the effect of potential protein partners on the activity of the glyceraldehyde-3-phosphate dehydrogenase (GAPDH)−CP12 preparation. It has also been used in standard assay conditions for phycocyanobilin:ferredoxin oxidoreductase (PcyA) enzyme assay.
Ferredoxin-NADP+ Reductase was used in in vitro ferredoxin-dependent desaturation of fatty acids in cyanobacterial thylakoid membranes. It was also used to regulate glyceraldehyde-3-phosphate dehydrogenase.

Biochem/physiol Actions

Ferredoxin-NADP+ reductase catalyzes the reversible conversion of reduced ferredoxin to oxidized ferredoxin during photosynthesis. Ferredoxin-NADP(H) reductase constitutes a family of hydrophilic FAD-containing monomeric enzymes that deliver NADPH or low potential one-electron donors to redox-based metabolisms in plastids, mitochondria, and bacteria.
Ferredoxin-NADP+ reductase catalyzes the reversible conversion of reduced ferredoxin to oxidized ferredoxin during photosynthesis. Ferredoxin-NADP(H) reductase constitutes a family of hydrophilic FAD-containing monomeric enzymes that deliver NADPH or low potential one-electron donors to redox-based metabolisms in plastids, mitochondria, and bacteria.

Unit Definition

One unit will reduce 1.0 millimole of cytochrome C per min at pH 7.5 at 25 °C in the presence of spinach ferredoxin and NADP.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Min Seok Cho et al.
Journal of microbiology (Seoul, Korea), 50(3), 496-501 (2012-07-04)
The Gram-positive bacterium Clavibacter michiganensis subsp. michiganensis is the causal agent of canker disease in tomato. Because it is very important to control newly introduced inoculum sources from commercial materials, the specific detection of this pathogen in seeds and seedlings
Malika Mekhalfi et al.
The New phytologist, 203(2), 414-423 (2014-05-07)
Diatoms are a widespread and ecologically important group of heterokont algae that contribute c. 20% to global productivity. Previous work has shown that regulation of their key Calvin cycle enzymes differs from that of the Plantae, and that in crude
Néstor Carrillo et al.
European journal of biochemistry, 270(9), 1900-1915 (2003-04-24)
Ferredoxin (flavodoxin)-NADP(H) reductases (FNR) are ubiquitous flavoenzymes that deliver NADPH or low potential one-electron donors (ferredoxin, flavodoxin) to redox-based metabolisms in plastids, mitochondria and bacteria. The plant-type reductase is also the basic prototype for one of the major families of
A Conserved Histidine-Aspartate Pair Is Required for Exovinyl Reduction of Biliverdin by a Cyanobacterial Phycocyanobilin:Ferredoxin Oxidoreductase
Tu SL, et al.
The Journal of Biological Chemistry, 281(6), 3127-3136 (2006)
[Laparocentesis in clinical traumatology].
V V Kuz'menko et al.
Khirurgiia, (6)(6), 50-54 (1986-06-01)

Protocols

Enzymatic Assay of Ferredoxin NADP Reductase - To standardize a procedure for the enzymatic assay of Ferredoxin NADP Reductase (EC.1.18.12).

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