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F9302

Sigma-Aldrich

Factor X Activated (Xa) from bovine plasma

aqueous glycerol solution

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Synonym(s):
Factor Xa
CAS Number:
Enzyme Commission number:
MDL number:
NACRES:
NA.54

form

aqueous glycerol solution

Quality Level

specific activity

≥50 units/mg protein

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

cow ... F10(280787)

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1 of 4

This Item
T7513T4648F8630
vibrant-m

T7513

Thrombin from bovine plasma

vibrant-m

T4648

Thrombin from bovine plasma

vibrant-m

F8630

Fibrinogen from bovine plasma

specific activity

≥50 units/mg protein

specific activity

≥2,000 NIH units/mg protein (E1%/280 = 19.5)

specific activity

40-300 NIH units/mg protein (biuret)

specific activity

-

Quality Level

200

Quality Level

200

Quality Level

300

Quality Level

300

shipped in

wet ice

shipped in

-

shipped in

wet ice

shipped in

-

form

aqueous glycerol solution

form

lyophilized powder

form

lyophilized powder

form

powder

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Application

Fusion proteins are commonly expressed with a factor Xa cleavable Ile-Glu (or Asp)-Gly-Arg-↓-X sequence. Typically 1 mg of fusion protein can be incubated with 10 μg of factor Xa for 2.5 hours at 37 °C.

Biochem/physiol Actions

Factor Xa catalyzes the hydrolysis of the Arg-Thr and then Arg-Ile bonds in prothrombin to yield active thrombin.
The fairly strict recognition sequence is Ile-Glu (or Asp)-Gly-Arg-↓-X.
It may sometimes cleave at other basic residues, depending on the conformation of the target protein. Factor Xa will not cleave if a proline residue follows the arginine of the recognition sequence.
pH Optimum: 7.6-8.0
Temperature Optimum: 37 °C

Physical properties

Factor Xa is a serine endoproteinase and a member of the S1 peptidase family. Factor Xa plays a critical role in the coagulation cascade by catalyzing the proteolytic conversion of prothrombin to active thrombin. Factor Xa′s prothrombin conversion activity is greatly enhanced in vivo when complexed with factor V, calcium ions and phospholipids on the activated platelet surface.
The zymogen form, Factor X, is activated in vivo by two different pathways. The intrinsic pathway utilizes a catalytic complex composed of factor IXa, factor VIII, phospholipids and calcium ions. The extrinsic pathway utilizes a complex of factor VII and tissue factor. The factor X zymogen is a 55 KDa glycoprotein with a light and heavy chain joined by a single disulfide.

Unit Definition

One unit of activated Factor X will liberate 1.0 μmole of p-nitroanilide from N-benzoyl-L-isoleucyl-L-glutamyl-L-glycyl-L-arginine-p-nitroaniline per minute at pH 8.3 at 37 °C.

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Substrate

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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Doron Bushi et al.
Journal of neuroscience research, 96(8), 1406-1411 (2018-05-16)
Factor Xa (FXa) plays a critical role in the coagulation cascade by generation of thrombin. During focal ischemia thrombin levels increase in the brain tissue and cause neural damage. This study examined the hypothesis that administration of the FXa inhibitor
[Xarelto: other advantages].
Perspective infirmiere : revue officielle de l'Ordre des infirmieres et infirmiers du Quebec, 10(1), 59-59 (2013-02-06)
Taki Galanis et al.
Hospital practice (1995), 41(1), 26-36 (2013-03-08)
Nonvalvular atrial fibrillation (AF) is the most common clinically significant cardiac arrhythmia occurring in patients in the United States. The primary clinical consequence of AF is an increase in the risk and severity of strokes. Treatment guidelines recommend anticoagulation therapy
Apixaban (Eliquis)--a new oral anticoagulant for atrial fibrillation.
The Medical letter on drugs and therapeutics, 55(1409), 9-10 (2013-02-06)
Antidotes edge closer to reversing effects of new blood thinners.
Elie Dolgin
Nature medicine, 19(3), 251-251 (2013-03-08)

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