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G6670

Sigma-Aldrich

Anti-Growth Factor Independence-1 antibody, Mouse monoclonal

clone 2.5D.17, purified from hybridoma cell culture

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Synonym(s):
Anti-GFI1, Anti-ZNF163
MDL number:
NACRES:
NA.41

biological source

mouse

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

2.5D.17, monoclonal

form

buffered aqueous solution

mol wt

antigen ~55 kDa

species reactivity

rat, mouse, human

concentration

~2 mg/mL

technique(s)

immunohistochemistry: suitable
microarray: suitable
western blot: 1-2 μg/mL using total cell extract of A549 cells

isotype

IgG1

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... GFI1(2672)
mouse ... Gfi1(14581)
rat ... Gfi1(24388)

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vibrant-m

C7855

Anti-Cdh1 antibody,Mouse monoclonal

species reactivity

rat, mouse, human

species reactivity

bovine, newt, chicken, human, monkey, mouse

species reactivity

human

species reactivity

rat, mouse, human

Gene Information

human ... GFI1(2672)
mouse ... Gfi1(14581)
rat ... Gfi1(24388)

Gene Information

human ... HNRNPL(3191)
mouse ... Hnrnpl(15388)

Gene Information

human ... CDH1(999)

Gene Information

human ... HDAC5(10014)
mouse ... Hdac5(15184)
rat ... Hdac5(84580)

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

clone

2.5D.17, monoclonal

clone

4D11, monoclonal

clone

DCS-266, monoclonal

clone

HDAC5-35, monoclonal

antibody form

purified from hybridoma cell culture

antibody form

purified from hybridoma cell culture

antibody form

purified from hybridoma cell culture

antibody form

purified from hybridoma cell culture

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General description

Monoclonal Anti-Growth Factor Independence-1 (GFI1) (mouse IgG1 isotype) is derived from the hybridoma 2.5D.17 produced by the fusion of mouse myeloma cells (SP2 cells) and splenocytes from BALB/c mice immunized with rat GFI1. Growth Factor Independence 1 (GFI1) is a zinc-finger transcriptional repressor, that is located on human chromosome 1p22. Gfi1 is highly expressed in thymus while Gfi1B is the predominant factor expressed in spleen, and both are expressed in bone marrow. It has a repression domain named SNAG at the N-terminal.† The protein also has a zinc finger DNA binding domain that is homologous to the domain in Gfi1B. Both bind to same consensus DNA sequence

Specificity

The antibody epitope resides within the GFI1 SNAG transcriptional-repression domain (amino acids 7-26).

Immunogen

rat growth factor independence-1 (GFI1).

application

Monoclonal Anti-Growth Factor Independence-1 (GFI1) antibody may be used in:
  • immunoblotting
  • immunohistochemistry
  • EMSA (electrophoretic mobility shift analysis)

Biochem/physiol Actions

Growth factor independence-1 (Gfi1) plays a key role in hematopoiesis along with Gfi1B proteins. Cytokines that regulate innate immunity induce Gfi1 expression. Deficiency of this protein causes thymic and peripherial T lymphopenia and severe abnormalities in pre-T-cell development in mice. Mutations in this gene cause defects in T lymphocyte function and development in humans. Gfi1 autoregulates its expression in T lymphocytes.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Analysis Note

The antibody may also be used in EMSA (electrophoretic mobility shift analysis).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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Loretta L Doan et al.
Journal of immunology (Baltimore, Md. : 1950), 170(5), 2356-2366 (2003-02-21)
T cell differentiation in the thymus is dependent upon signaling through the TCR and is characterized by the resulting changes in expression patterns of CD4 and CD8 surface coreceptor molecules. Although recent studies have characterized the effects of proximal TCR
Targeted transcriptional repression of Gfi1 by GFI1 and GFI1B in lymphoid cells
Doan LL, et al.
Nucleic Acids Research, 32(8), 2508-2519 (2004)
Chinavenmeni S Velu et al.
Blood, 113(19), 4720-4728 (2009-03-13)
The zinc finger protein growth factor independent-1 (Gfi1) is a transcriptional repressor that is critically required for normal granulocytic differentiation. GFI1 loss-of-function mutations are found in some patients with severe congenital neutropenia (SCN). The SCN-associated GFI1-mutant proteins act as dominant
Cloning of the human Gfi-1 gene and its mapping to chromosome region 1p22
Roberts T and Cowell JK
Oncogene, 14(8), 1003-1003 (1997)
The downregulation of GFI1 by the EZH2-NDY1/KDM2B-JARID2 axis and by human cytomegalovirus (HCMV) associated factors allows the activation of the HCMV major IE promoter and the transition to productive infection
Sourvinos G, et al.
PLoS Pathogens, 10(5), e1004136-e1004136 (2014)

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