G9277
Guanidine thiocyanate
for molecular biology, ≥99%
Recommended Products
biological source
synthetic (organic)
Quality Level
grade
for molecular biology
assay
≥99%
form
powder or crystals
storage condition
(Tightly closed. Dry. )
technique(s)
RNA extraction: suitable
color
colorless to white,
mp
120-122 °C (lit.)
solubility
water: 100 mg/mL, clear, colorless
density
1.29 g/cm3 at 20 °C (68 °F)
cation traces
Ba: ≤5 ppm
Fe: ≤5 ppm
Na: ≤0.5%
heavy metals (as Pb): ≤5 ppm
application(s)
agriculture
foreign activity
DNase, RNase, none detected
SMILES string
SC#N.NC(N)=N
InChI
1S/CH5N3.CHNS/c2-1(3)4;2-1-3/h(H5,2,3,4);3H
InChI key
ZJYYHGLJYGJLLN-UHFFFAOYSA-N
Looking for similar products? Visit Product Comparison Guide
General description
In nucleic acid purification, guanidine thiocyanate is effective in inactivating DNases and RNases, preventing nucleic acid degradation and ensuring favorable yields. Its dual denaturing and enzyme-inhibiting properties make it crucial for DNA and RNA studies. Beyond nucleic acid isolation, guanidine thiocyanate is used for cell lysis and virus particle inactivation during RNA and DNA extractions. It not only disrupts membranes but also denatures RNase and DNase enzymes, protecting extracted nucleic acids from enzymatic degradation. The versatility of guanidine thiocyanate extends to molecular biology techniques like PCR, Northern blotting, and Southern blotting. It serves as a vital reagent in these methods, contributing to the successful analysis and amplification of nucleic acids.
Application
- for the lysis of colorectal adenocarcinoma cell line prior to RNA isolation
- as a PCR inhibitor in triplex mitochondrial DNA (mtDNA) qPCR assay
- for DNA extraction from cerebrospinal fluid (CSF)
Biochem/physiol Actions
Features and Benefits
- Highly versatile surfactant for your cell biology and biochemical research
- Suitable for RNA extraction in sensitive molecular biology applications
- Free from Dnase and Rnase
- Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications
Other Notes
comparable product
signalword
Danger
Hazard Classifications
Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Skin Corr. 1C
supp_hazards
Storage Class
8B - Non-combustible, corrosive hazardous materials
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service