Skip to Content
MilliporeSigma
All Photos(1)

Documents

GE17-0090-02

Sephadex® LH-20

Cytiva 17-0090-02, pack of 500 g

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
23151817
NACRES:
NA.56

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

feature

autoclavable 20 min at 121°C

packaging

pack of 500 g

manufacturer/tradename

Cytiva 17-0090-02

matrix

hydroxypropylated, cross-linked dextran

particle size

163 μm (in methanol max.)
18-111 μm (dry)
27 μm (In Methanol Min.)

cleaning in place

2-13

working range

2-13

General description

Sephadex® LH20 is a liquid chromatography media for molecular sizing of natural product such as steroid, terpenoid, lipid, low molecular weight peptide. Useful to analytical and industrial scale for preparation of closely related molecular species.

Sephadex® LH-20 is prepared by hydroxypropylation of Sephadex® G-25, a bead-formed dextran medium, and has been specifically developed for gel filtration of natural products, such as steroids, terpenoids, lipids and low molecular weight peptides, in organic solvents.

Sephadex® LH-20 is useful at both analytical and industrial scale for the preparation of closely related molecular species. Due to the physico-chemical properties of this media, it can be used either during initial purification prior to polishing by high performance ion exchange or reversed phase chromatography or as the final polishing step e.g. during the preparation of diastereomers. Sephadex® LH-20 is characterized by (1) Chromatographic selectivity due to dual hydrophilic and lipophilic nature of the matrix (2) Easily predicted elution behaviour based on the chemical structure 1–3, 16 of the sample (3) Chemical and physical robustness (4) Excellent batch to batch reproducibility.

Features and Benefits

  • Designed for use in polar organic solvents and aqueous solvent mixtures
  • Excellent for fractionation of lipids, hormones, vitamins, and other small biomolecules.
  • Useful for both analytical and industrial scales for the preparation of closely related molecular species
  • Sephadex® LH-20 is made from hydroxypropylated dextran beads that have been crosslinked to yield a polysaccharide network.
  • Powerful chromatographic selectivity due to dual hydrophilic and lipophilic nature of the matrix
  • Excellent batch-to-batch reproducibility
  • Sephadex® LH-20 is made from hydroxypropylated dextran beads that have been crosslinked to yield a polysaccharide network.

Storage and Stability

Store at 4 to 25 °C ()

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Sephadex is a registered trademark of Cytiva

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Customers Also Viewed

Slide 1 of 2

1 of 2

Sephadex® G-50 Medium

Supelco

G50150

Sephadex® G-50

Sephadex® G-50 Fine, Cytiva, pack of 100 g

GE17-0042-01

Sephadex® G-50

Rajini Nagaraj et al.
Nanomaterials (Basel, Switzerland), 10(3) (2020-04-03)
Diabetic retinopathy (DR), Retinopathy of Pre-maturity (ROP), and Age-related Macular Degeneration (AMD) are multifactorial manifestations associated with abnormal growth of blood vessels in the retina. These three diseases account for 5% of the total blindness and vision impairment in the

Protocols

Separation using Sephadex LH-20 column for Size Exclusion Chromatography media in polysaccharide network from Cytiva.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service