GE17-0405-03
HiTrap® Protein G High Performance
Cytiva 17-0405-03, pack of 5 × 5 mL
Recommended Products
ligand
recombinant protein G lacking albumin-binding region
shelf life
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging
pack of 5 × 5 mL
manufacturer/tradename
Cytiva 17-0405-03
storage condition
(20% Ehtanol)
(20% Ethanol)
parameter
42 psi
bed size
16 mm × 25 mm
bed volume
5 mL
column I.D.
16 mm
size
avg. part. size
34 μm
cleaning
2-9(pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.)
working range
3-9(pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.)
capacity
>1 mg binding capacity (lgG/column)(The binding capacity has been determined with human polyclonal IgG.)
125 mg binding capacity(human IgG/column)
storage temp.
2-8°C
Related Categories
General description
Features and Benefits
- High binding capacity of 25 mg IgG/mL medium and fast binding kinetics.
- 34 μm bead size for high performance, high-resolution purifications.
- Binds broad range of different species and subclasses of IgG.
- Simple and proven method giving reproducible results.
- Designed for use with syringe, peristalic pump, or chromatography system.
Analysis Note
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Certificates of Analysis (COA)
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Articles
Purify monoclonal or polyclonal IgG from serum, cell culture supernatant or ascitic fluid using the HiTrap Protein G HP from Cytiva, an affinity-exclusion chromatography product containing Sepharose-immobilized Protein G.
This page shows how to purify IgM antibodies by affinity chromatography using HiTrap™ IgM Purification HP.
Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.
This page shows how to solve practical problems that may occur when running an affinity chromatography column.
Protocols
This page shows how to perform a two-step purification of mouse monoclonal IgG1 using HiTrap® Protein G HP from Cytiva for the capture step.
General instructions for affinity purification using HiTrap columns from Cytiva.
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