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GE17-0618-02

Protein G Sepharose 4 Fast Flow

Cytiva 17-0618-02, pack of 25 mL

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NACRES:
NA.56

ligand

recombinant protein G lacking albumin-binding region

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

packaging

pack of 25 mL

manufacturer/tradename

Cytiva 17-0618-02

storage condition

(20% Ehtanol)

matrix

4% cross-linked agarose

average diameter

90 μm (d50v)

cleaning in place

2-10

working range

3-9

suitability

suitable for bioprocess medium

storage temp.

2-8°C

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GE17-0618-01GE17-5280-01GE17-5132-02
matrix

4% cross-linked agarose

matrix

4% cross-linked agarose

matrix

4% cross-linked agarose

matrix

highly cross-linked 4% agarose

packaging

pack of 25 mL

packaging

pack of 5 mL

packaging

pack of 5 mL

packaging

pack of 100 mL

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

-

storage condition

(20% Ehtanol)

storage condition

(20% Ehtanol)

storage condition

(20% Ehtanol)

storage condition

-

suitability

suitable for bioprocess medium

suitability

suitable for bioprocess medium

suitability

suitable for bioprocess medium

suitability

-

General description

Protein G Sepharose 4 Fast Flow is recombinant protein G coupled to Sepharose 4 Fast Flow.

Protein G Sepharose 4 Fast Flow has recombinant protein G immobilized by the cyanogen bromide (CNBr) method to Sepharose 4 Fast Flow. Protein G exhibit binding specificities that complement Protein A media and binds to the Fc region of IgG from a variety of mammalian species. Protein G Sepharose 4 Fast Flow may be used to isolate and purify classes, subclasses and fragments of immunoglobulins from any biological fluid or cell culture medium.

As member of the BioProcess media range, Protein G Sepharose 4 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support.

Features and Benefits

  • Binding specificities that complement Protein A media.
  • Binds a broad range of IgG species and subclasses.
  • Multi-point attachment minimizes ligand leakage.
  • Used in a range of research applications.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Sepharose is a trademark of Cytiva

pictograms

Flame

signalword

Warning

hcodes

Storage Class

3 - Flammable liquids


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25G
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705578-5MG-PW

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MMYOMAG-74K-13

1000309185

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Monoclonal antibodies (mAbs) are a focus in vaccine and therapeutic design to counteract severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and its variants. Here, we combined B cell sorting with single-cell VDJ and RNA sequencing (RNA-seq) and mAb structures to

Articles

Purify monoclonal or polyclonal IgG from serum, cell culture supernatant or ascitic fluid using the HiTrap Protein G HP from Cytiva, an affinity-exclusion chromatography product containing Sepharose-immobilized Protein G.

This page describes immunoprecipitation (immunoaffinity or pull-down techniques).

This page describes efficient column packing and preparation for affinity chromatography of antibodies.

This page shows how to convert between flow velocity and volumetric flow rate in affinity chromatography of antibodies.

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Protocols

This page provides information about different pull-down assays for the further isolation of multiprotein complexes to identify their components with products from Cytiva.

This page shows how to separate IgG antibodies by affinity chromatography using Protein G Sepharose 4 Fast Flow from Cytiva.

This page shows how to prepare samples for purification with affinity chromatography.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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