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GE17-0719-01

CM Sepharose Fast Flow

Cytiva 17-0719-01, pack of 500 mL

Synonym(s):

CM Sepharose, Fast Flow CM Resin

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About This Item

UNSPSC Code:
41106500
NACRES:
NA.56

ligand

carboxymethyl

description

Ion Exchanger Type (value)

packaging

pack of 500 mL

manufacturer/tradename

Cytiva 17-0719-01

matrix

6% cross-linked agarose

particle size

45-165 μm

average diameter

90 μm

cleaning in place

2-14

working range

4-13

suitability

suitable for bioprocess medium

General description

CM Sepharose Fast Flow is a weak cation exchanger based on the well established Sepharose Fast Flow ion exchange platform, extensively used for preparative protein separations in both research and industrial applications.

CM Sepharose Fast Flow is part of the Sepharose Fast Flow ion exchange platform, which has been the industrial standard for ion exchange chromatography during recent decades. It is composed of crosslinked 6% agarose beads, with carboxymethyl (CM) weak cation exchange groups. CM Sepharose Fast Flow has high chemical stability, allowing well proven cleaning-in-place (CIP) and sanitization protocols. Scale-up with CM Sepharose Fast Flow is straight forward and the medium is available in a range of pre-packed process development tools.

As member of the BioProcess media range, CM Sepharose Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support.

Features and Benefits

  • Well-proven weak cation exchanger developed for industrial downstream processes
  • Used extensively for capture and intermediate purification of a wide range of approved biopharmaceuticals
  • The industry standard for ion exchange chromatography during recent decades
  • High chemical stability allows for well proven CIP and sanitization protocols
  • The hydrophilic nature of the base matrix ensures low levels of non-specific binding leading to low levels of host cell-derived impurities in the elution pool.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Sepharose is a trademark of Cytiva

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Warning

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Storage Class

3 - Flammable liquids


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.

This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.

This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.

This page covers detailed aspects of each step in an IEX separation to improve resolution and overall performance.

Protocols

This page covers the use of Sepharose Fast Flow for purification of proteins.

This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from Cytiva.

This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

This page covers detailed information on cleaning procedures and recommended flow for column cleaning.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service