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GE17-5248-02

HisTrap High Performance

Cytiva 17-5248-02, pack of 5 × 5 mL

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Synonym(s):
Nickel-charged chromatography column, HP columns, IMAC-columns, protein purification columns
NACRES:
NA.56

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

packaging

pack of 5 × 5 mL

manufacturer/tradename

Cytiva 17-5248-02

parameter

4 ml/min (1 ml), 5 ml/min (5ml) flow rate (H2O at room temperature)
42 psi (H2O at room temperature.)

bed size

16 mm × 25 mm

bed volume

5 mL

column I.D.

16 mm

matrix

highly cross-linked 6% agarose

avg. part. size

34 μm

cleaning

2-14(Ni2+-stripped medium.)

working range

3-12(Ni2+-stripped medium.)

capacity

≥40 mg binding capacity (histidine-tagged protein/ml medium)(H20 at room temperature)
≥40 mg binding capacity (histidine-tagged protein/ml medium)(Protein binding capacity is protein-to-protein dependent.)

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This Item
GE29-0510-21GE17-5281-01GE17-5255-01
HisTrap™ High Performance Cytiva 17-5248-02, pack of 5 × 5 mL

GE17-5248-02

HisTrap High Performance

HisTrap™ High Performance Cytiva 29-0510-21, pack of 1 mL

GE29-0510-21

HisTrap High Performance

GSTrap™ High Performance Cytiva 17-5281-01, pack of 5 × 1 mL

GE17-5281-01

GSTrap High Performance

HisTrap™ Fast Flow Cytiva 17-5255-01, pack of 5 × 5 mL

GE17-5255-01

HisTrap Fast Flow

matrix

highly cross-linked 6% agarose

matrix

highly cross-linked 6% agarose

matrix

highly cross-linked 6% agarose

matrix

6% cross-linked agarose

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

manufacturer/tradename

Cytiva 17-5248-02

manufacturer/tradename

Cytiva 29-0510-21

manufacturer/tradename

Cytiva 17-5281-01

manufacturer/tradename

Cytiva 17-5255-01

parameter

4 ml/min (1 ml), 5 ml/min (5ml) flow rate (H2O at room temperature)

parameter

flow rate, 4 ml/min (1 ml), 5 ml/min (5ml) flow rate (H2O at room temperature), 42 psi (H2O at room temperature.)

parameter

<4 mL/min flow rate (The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.), 42 psi (H2O at room temperature.)

parameter

<4 mL/min flow rate (The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.), 42 psi

bed size

16 mm × 25 mm

bed size

7 mm × 25 mm

bed size

7 mm × 25 mm

bed size

7 mm × 25 mm

General description

HisTrap HP columns are prepacked with Ni Sepharose High Performance and designed for simple, high-resolution purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).

HisTrap HP 1 mL and 5 mL columns are designed for simple, one-step purification of histidine-tagged proteins. The columns are prepacked with Ni Sepharose High Performance, which has high binding capacity and low nickel ion leakage that ensures reliable capture of target protein in repeated IMAC purifications.

HisTrap HP columns can also be used for the purification of tagged proteins containing shorter or longer polyhistidine tags, such as (histidine)4 or (histidine)10. The shorter (histidine)4 will bind more weakly and the longer (histidine)10 will bind more strongly compared with (histidine)6. This difference in binding strength can be used during purification; since (histidine)10 binds more strongly, a higher concentration of imidazole can be added to the lysed cells. This can facilitate the removal of contaminants that can otherwise be copurified with the tagged target protein.

The high stability and broad compatibility of Ni Sepharose High Performance maintains biological activity and increases product yield, at the same time as it greatly expands the range of suitable operating conditions.

For convenient scaling up of histidine-tagged protein purification, use 20 mL HisPrep FF 16/10 columns. Ni Sepharose 6 Fast Flow, the medium prepacked in HisTrap FF and HisPrep FF 16/10 columns, allows high flow rates, which facilitates scale-up of histidine-tagged protein purification.

Features and Benefits

  • High binding capacity, at least 40 mg/mL chromatography medium.
  • Compatible with a wide range of reducing agents, detergents, denaturants, and other additives.
  • Negligible Ni2+ leakage
  • Simple manual operation with a syringe, pump, or chromatography system such as AKTA design

Storage and Stability

Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

HisPrep is a trademark of Cytiva
HisTrap is a trademark of Cytiva
Sepharose is a trademark of Cytiva

Pictograms

Environment

Signal Word

Warning

Hazard Statements

Storage Class Code

3 - Flammable liquids


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Articles

This page shows how to perform a purification and on-column refolding of an insoluble his-tagged protein from an E. coli culture with HisTrap™ FF columns and ÄKTAprime from Cytiva.

This page shows high-throughput screening using His MultiTrap™ HP and His MultiTrap™ FF 96-well filter plates from Cytiva.

This page describes principles and standard conditions for different purification techniques of histidine-tagged proteins using Cytiva products.

Protocols

This page shows how to purify Strep-tag II recombinant proteins using StrepTrap HP 1 mL and 5 mL from Cytiva.

Ni Sepharose High Performance consists of highly cross-linked 6% agarose beads (34 µm) to which a chelating group has been immobilized and subsequently charged with Ni2+ ions.

This page shows how to purify protein A-tagged proteins using Cytiva products.

How to isolate proteins from inclusion bodies using Cytiva products.

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