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GE17-5255-01

HisTrap Fast Flow

Cytiva 17-5255-01, pack of 5 × 5 mL

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Synonym(s):
HisTrap FF, His-tagged protein purification column, ready-to-use column
NACRES:
NA.56

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

packaging

pack of 5 × 5 mL

manufacturer/tradename

Cytiva 17-5255-01

parameter

<4 mL/min flow rate (The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.)
42 psi

bed size

7 mm × 25 mm

bed volume

1 mL

column I.D.

7 mm

matrix

6% cross-linked agarose

particle size

45-165 μm

average diameter

90 μm

cleaning

2-14(Ni2+-stripped medium.)

working range

3-12(Ni2+-stripped medium.)

capacity

~40 mg binding capacity(histidine-tagged protein)

suitability

suitable for bioprocess medium

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matrix

6% cross-linked agarose

matrix

6% cross-linked agarose

matrix

6% cross-linked agarose

matrix

6% cross-linked agarose

particle size

45-165 μm

particle size

45-165 μm

particle size

45-165 μm

particle size

45-165 μm

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

manufacturer/tradename

Cytiva 17-5255-01

manufacturer/tradename

Cytiva 17-5319-01

manufacturer/tradename

Cytiva 17-5286-01

manufacturer/tradename

Cytiva 17-5157-01

parameter

<4 mL/min flow rate (The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.)

parameter

<20 mL/min flow rate, 42 psi

parameter

<20 mL/min flow rate, 42 psi (H2O at room temperature.)

parameter

flow rate, <20 mL/min flow rate (The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.), 42 psi

General description

HisTrap FF is a ready-to-use column, prepacked with precharged Ni Sepharose 6 Fast Flow for preparative purification of histidine-tagged recombinant proteins by immobilized metal ion affinity chromatography (IMAC).

Ni Sepharose 6 Fast Flow consists of 90 μm beads of highly cross-linked agarose, to which a chelating ligand has been immobilized. This chelating ligand is charged with Ni2+ ions, the first-choice metal ion for purifying most histidine-tagged proteins. The negligible leakage of Ni2+ ions from the matrix ensures reliable capture of histidine-tagged proteins in repeated IMAC purifications.

The high flow rates made possible by the Ni Sepharose 6 Fast Flow matrix make HisTrap FF columns well suited for small scale-up. Capacity can be increased by connecting columns in series. HiTrap® IMAC FF is the product of choice when charging the medium with different metal ions for optimization of purification protocols.

Features and Benefits

  • High binding capacity, approx. 40 mg/mL medium.
  • Negligible leakage of Ni2+.
  • Prepacked columns offer reliable and convenient time-saving purification of histidinetagged recombinant proteins.
  • Compatible with a wide range of reducing agents, detergents, denaturants, and other additives.

Storage and Stability

Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

HiTrap is a registered trademark of Cytiva
HisTrap is a trademark of Cytiva
Sepharose is a trademark of Cytiva

Pictograms

Environment

Signal Word

Warning

Hazard Statements

Storage Class Code

3 - Flammable liquids


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Articles

This page shows high-throughput screening using His MultiTrap™ HP and His MultiTrap™ FF 96-well filter plates from Cytiva.

How to optimize purification of histidine-tagged proteins using Cytiva products.

This page describes principles and standard conditions for different purification techniques of histidine-tagged proteins using Cytiva products.

This page shows troubleshooting instructions for affinity chromatography of tagged proteins using Cytiva products.

Protocols

This page shows how to perform a cell disruption und prepare membranes with products from Cytiva.

This page shows the purification strategy of capture, intermediate purification, and polishing (CIPP) in affinity chromatography using Cytiva products.

This page shows how to purify protein A-tagged proteins using Cytiva products.

This page shows how to purify histidine-tagged recombinant proteins using Ni Sepharose 6 Fast Flow from Cytiva. Ni Sepharose 6 Fast Flow consists of 90 µm beads of highly cross-linked agarose, to which a chelating ligand has been immobilized and subsequently charged with Ni2+ ions.

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