GE28-9520-96
HiTrap® Con A 4B
Cytiva 28-9520-96, pack of 5 × 5 mL
About This Item
Recommended Products
ligand
concanavalin A (con A)
packaging
pack of 5 × 5 mL
manufacturer/tradename
Cytiva 28-9520-96
storage condition
(100% Isopropanol)
(20% Ethanol containing 0.1 M Acetate Buffer pH 6, 1 M NaCl, 1 mM CaCl2, 1 mM MnCl2 and 1 mM MgCl2)
parameter
42 psi
bed size
16 mm × 25 mm
bed volume
5 mL
column I.D.
16 mm
matrix
4% agarose
particle size
45-165 μm
average diameter
90 μm
cleaning
4-9
working range
4-6
storage temp.
2-8°C
Related Categories
General description
Concanavalin A (Con A) is a tetrameric metalloprotein isolated from Canavalia ensiformis (jack bean). Con A binds molecules containing α-D-mannopyranosyl, α-D-glucopyranosyl, and sterically related residues. The binding sugar requires the presence of C-3, C-4 and C-5 hydroxyl groups for reaction with Con A. Con A coupled to Sepharose™ is routinely used for separation and purification of glycoproteins, polysaccharides, and glycolipids.
Features and Benefits
- Con A Sepharose™ is concanavalin A coupled to Sepharose™ 4B.
- HiTrap® Con A 4B columns can be operated with a syringe, a peristaltic pump, or a liquid chromatography system such as AKTA design.
Storage and Stability
Analysis Note
Other Notes
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Warning
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Storage Class
3 - Flammable liquids
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Articles
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Protocols
This page shows how to purify or remove glycoproteins and polysaccharides with Con A Sepharose 4B, Lentil Lectin Sepharose 4B, Capto Lentil Lectin from Cytiva.
This page covers detailed information on cleaning procedures and recommended flow for column cleaning.
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