Skip to Content
MilliporeSigma
All Photos(1)

Documents

GE28-9520-96

HiTrap® Con A 4B

Cytiva 28-9520-96, pack of 5 × 5 mL

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
23151816
NACRES:
NA.56

ligand

concanavalin A (con A)

shelf life

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

packaging

pack of 5 × 5 mL

manufacturer/tradename

Cytiva 28-9520-96

storage condition

(100% Isopropanol)
(20% Ethanol containing 0.1 M Acetate Buffer pH 6, 1 M NaCl, 1 mM CaCl2, 1 mM MnCl2 and 1 mM MgCl2)

parameter

42 psi

bed size

16 mm × 25 mm

bed volume

5 mL

column I.D.

16 mm

matrix

4% agarose

particle size

45-165 μm

average diameter

90 μm

cleaning

4-9

working range

4-6

storage temp.

2-8°C

Related Categories

General description

HiTrap® Con A 4B is a ready-to-use column prepacked with Con A Sepharose 4B, a medium for convenient separation and purification of glycoproteins, polysaccharides, and glycolipids.

Concanavalin A (Con A) is a tetrameric metalloprotein isolated from Canavalia ensiformis (jack bean). Con A binds molecules containing α-D-mannopyranosyl, α-D-glucopyranosyl, and sterically related residues. The binding sugar requires the presence of C-3, C-4 and C-5 hydroxyl groups for reaction with Con A. Con A coupled to Sepharose is routinely used for separation and purification of glycoproteins, polysaccharides, and glycolipids.

Features and Benefits

  • Con A Sepharose is concanavalin A coupled to Sepharose 4B.
  • HiTrap® Con A 4B columns can be operated with a syringe, a peristaltic pump, or a liquid chromatography system such as AKTA design.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Other Notes

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Legal Information

HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Customers Also Viewed

Slide 1 of 1

1 of 1

HiTrap® Con A 4B Cytiva 28-9520-85, pack of 5 × 1 mL

GE28-9520-85

HiTrap® Con A 4B

Articles

This page shows how to convert between flow velocity and volumetric flow rate in affinity chromatography of antibodies.

Protocols

This page shows how to purify or remove glycoproteins and polysaccharides with Con A Sepharose 4B, Lentil Lectin Sepharose 4B, Capto Lentil Lectin from Cytiva.

This page covers detailed information on cleaning procedures and recommended flow for column cleaning.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service