GE28-9520-96

HiTrap^® Con A 4B

Cytiva 28-9520-96, pack of 5 × 5 mL

• UNSPSC Code: 23151816
• NACRES: NA.56

Properties

• ligand: concanavalin A (con A)
• packaging: pack of 5 × 5 mL
• manufacturer/tradename: Cytiva 28-9520-96
• storage condition: (100% Isopropanol); (20% Ethanol containing 0.1 M Acetate Buffer pH 6, 1 M NaCl, 1 mM CaCl2, 1 mM MnCl2 and 1 mM MgCl2)
• parameter: 42 psi
• bed size: 16 mm × 25 mm
• bed volume: 5 mL
• column I.D.: 16 mm
• matrix: 4% agarose
• particle size: 45-165 μm
• average diameter: 90 μm
• cleaning: 4-9
• working range: 4-6
• storage temp.: 2-8°C

Description

General description

HiTrap^® Con A 4B is a ready-to-use column prepacked with Con A Sepharose^™ 4B, a medium for convenient separation and purification of glycoproteins, polysaccharides, and glycolipids.

Concanavalin A (Con A) is a tetrameric metalloprotein isolated from Canavalia ensiformis (jack bean). Con A binds molecules containing α-D-mannopyranosyl, α-D-glucopyranosyl, and sterically related residues. The binding sugar requires the presence of C-3, C-4 and C-5 hydroxyl groups for reaction with Con A. Con A coupled to Sepharose^™ is routinely used for separation and purification of glycoproteins, polysaccharides, and glycolipids.

Features and Benefits

• Con A Sepharose^™ is concanavalin A coupled to Sepharose^™ 4B.
• HiTrap^® Con A 4B columns can be operated with a syringe, a peristaltic pump, or a liquid chromatography system such as AKTA design.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Other Notes

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Legal Information

HiTrap is a registered trademark of Cytiva

Sepharose is a trademark of Cytiva

Safety Information

• pictograms: GHS02
• signalword: Warning
• hcodes: H226
• pcodes: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• Storage Class: 3 - Flammable liquids