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MilliporeSigma

GE28-9889-49

XK 26/40 Column

Synonym(s):

XK Column

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About This Item

UNSPSC Code:
41115711
NACRES:
SB.52

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technique(s)

LPLC: suitable

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General description

XK empty columns provide reliable and reproducible separations. The tube material is of borosilicate glass, which allows for visual inspection of the media bed. The columns are made of biocompatible materials that exhibit excellent chemical resistance. A broad range of column dimensions makes XK columns suitable for many different purification needs. Column packing can be performed using either a packing reservoir or an extra column tube attached with a packing connector. The QuickLock mechanism of the adapter shaft facilitates rapid and easy movement of the adapter, simplifying adjustments of the bed height, disassembly, and cleaning. The design of the adapter plunger gives a uniform flow, which maintains the integrity of the packed bed during operations. An extensive range of spare parts is available, facilitating maintenance.

Features and Benefits

Adapter QuickLock mechanism that facilitates column handling and cleaning
Cooling jacket for sensitive purifications
Fittings for direct connection to chromatographiy systems
Plunger design to ensure a homogeneous buffer distribution and reproducible packing
User friendly and robust empty columns, designed for liquid chromatography at low to medium pressure.

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Articles

Desalting and Buffer Exchange for Affinity Chromatography of Antibodies

Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.

Column Packing and Preparation for Affinity Chromatography of Antibodies

This page describes efficient column packing and preparation for affinity chromatography of antibodies.

Converting from Flow Velocity to Volumetric Flow Rates

This page shows how to convert between flow velocity and volumetric flow rate in affinity chromatography of antibodies.

Practical Considerations for IEX Separation

This page covers detailed aspects of each step in an IEX separation to improve resolution and overall performance.

Protocols

Sample Preparation in Ion Exchange Chromatography

This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

Column Packing and Preparation

This page describes efficient column packing and preparation for affinity chromatography of tagged proteins using Cytiva products.

Column Packing and Preparation for Affinity Chromatography of GST-tagged Proteins

This page describes efficient column packing and preparation for affinity chromatography of GST-tagged proteins using Cytiva products.

Column packing and preparation for Ion Exchange Chromatography & Chromatafocusing

This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from Cytiva.

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