GE29-0181-84
HiPrep™ Phenyl High Performance16/10
Cytiva 29-0181-84, pack of 1 column
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About This Item
Recommended Products
ligand
phenyl
packaging
pack of 1 column
manufacturer/tradename
Cytiva 29-0181-84
parameter
1-5 ml/min flow rate
44 psi
bed size
16 mm × 100 mm
column I.D.
16 mm
matrix
6% cross-linked agarose
particle size
24-44 μm
avg. part. size
34 μm
cleaning
2-14
working range
3-13
suitability
suitable for bioprocess medium (BioProcess medium.)
suitable for bioprocess medium
General description
HiPrep 16/10 Phenyl HP is a prepacked column designed for preparative hydrophobic interaction chromatography (HIC) with good resolution. Phenyl Sepharose HP chromatography resin is based on a matrix of highly cross-linked agarose beads, which have been derivatized with phenyl groups. These are attached to the agarose matrix via uncharged, chemically stable ether linkages. The result is a truly hydrophobic resin with no ionic properties. High mechanical strength of the matrix allows for high flow rates, together with good resolution. Capacities vary according to the protein applied and its concentration. Loading capacity will also vary depending on the salt concentration, pH, and temperature at which the protein is applied.
Features and Benefits
- Aromatic HIC resin for intermediate purification and polishing.
- Selectivity similar to Phenyl Sepharose 6 Fast Flow (Low Sub) but higher resolution due to the smaller 34 µm bead size.
- Provides fast, easy high-capacity separations in a convenient format.
Storage and Stability
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Other Notes
The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.
Legal Information
HiPrep is a trademark of Cytiva
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Storage Class
3 - Flammable liquids
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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