Skip to Content
MilliporeSigma
All Photos(1)

Key Documents

GERPN2134

ECL™ Western Blotting Reagents

Cytiva RPN2134

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

UNSPSC Code:
41105332
NACRES:
NA.32

feature

wetted part: no

packaging

pkg of 1 kit

manufacturer/tradename

Cytiva RPN2134

color

Detection reagent 1: Colorless Detection reagent 2: Colorless

storage temp.

2-8°C

Related Categories

General description

Amersham™ ECL™ Western Blotting Detection Reagents were the world′s first commercial chemiluminescent reagents for Western blotting and are still the most widely used. They are at least 10-fold more sensitive than colorimetric assays and produce excellent and reliable results every time.

These reagents use enhanced luminol-based detection which is suitable for all routine western blotting. ECL™ Western Blotting Detection Reagents in combination with Hybond® ECL™ membranes form a complete solution for Western blot applications.

Contents include the following:
GERPN2109, 62.5 mL each of ECL™ Detection Reagents 1 and 2 (1000 cm2)
GERPN2209, 125 mL each (2000 cm2)
GERPN2106, 250 mL each (4000 cm2)
GERPN2134, 375 mL each (6000 cm2)

Application

ECL™ Western Blotting Reagents have been used in Western blotting.

Features and Benefits

  • High sensitivity, ~10 pg in model system.
  • Proven performance, the most frequently cited ECL™ Western Blotting Detection Reagents in publications
  • Detectable on Hyperfilm ECL™ or with CCD imager
  • Recommended membrane Hybond ECL™

Components

The kit contains
3 x 125 mL of ECL™ Detection reagent 1
3 x 125 mL of ECL™ detection reagent 2

Sufficient for detection of 6000 cm2 of membrane:
375 mL Detection reagent 1(color : colorless)
375 mL Detection reagent 2 (color: colorless)

Storage and Stability

Please be aware this product may be shipped 30 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Amersham is a trademark of Cytiva
ECL is a trademark of Cytiva
Hybond is a registered trademark of Cytiva

Storage Class

10 - Combustible liquids

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

CD4+ T cell responses in Balb/c mice with food allergy induced by trinitrobenzene sulfonic acid and ovalbumin.
Sun CY, et al.
Molecular Medicine Reports, 13(6), 5349-5357 (2016)

Articles

Methods Used to Detect Proteins and Nucleic Acids Bound to Membranes

Background and protocols describing the various methods used by molecular biologists to detect samples of protein or nucleic acids bound to membranes.

Troubleshooting Detection Methods for GST-tagged Proteins

This page describes troubleshooting strategies in the detection of GST-tagged proteins.

Protocols

Chemiluminescence Detection with Amersham ECL, Amersham ECL Prime, and Amersham ECL Select

This page describes chemiluminescence detection of GST-tagged proteins with Amersham ECL, Amersham ECL Prime, and Amersham ECL Select from Cytiva.

Detection of GST-tagged Proteins with Western Blot

This page describes how to monitor expression and purification of GST-tagged proteins by Western blot analysis using Cytiva products.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service