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H4281

SAFC

EX-CELL® Hybridoma Medium

without antibiotics, antimycotics, L-glutamine, and phenol red, with sodium bicarbonate, HEPES, essential and non-essential amino acids, liquid, sterile-filtered, suitable for hybridoma

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Synonym(s):
Hybridoma Medium
MDL number:
MFCD03098239
NACRES:
NA.75

description

endotoxin tested
for research or for further manufacturing use

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | hybridoma: suitable

components

HEPES: yes
NaHCO3: yes
L-glutamine: no
phenol red: no
glucose: 2.9 g/L

shipped in

ambient

storage temp.

2-8°C

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H4281

EX-CELL® Hybridoma Medium

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EX-CELL® CD Hybridoma Medium, Chemically Defined

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24621C

EX-CELL® 620-HSF Serum-Free Medium for Hybridoma Cells

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14610C

EX-CELL® 610-HSF Serum-Free Medium for Hybridoma Cells

form

liquid

form

liquid

form

dry powder

form

liquid

technique(s)

cell culture | hybridoma: suitable

technique(s)

cell culture | hybridoma: suitable, cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | hybridoma: suitable, cell culture | mammalian: suitable

components

HEPES: yes
NaHCO3: yes
L-glutamine: no
phenol red: no
glucose: 2.9 g/L

components

glucose: 2.84 g/L
NaHCO3: yes
L-glutamine: no

components

-

components

glucose: 3.45 g/L
NaHCO3: 1.60 g/L
L-glutamine: 0.333 g/L
HEPES: 7.5 mM

shipped in

ambient

shipped in

ambient

shipped in

-

shipped in

ambient

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

Application

Serum-free, low-protein medium formulated to support the growth of hybridomas used in the manufacture of monoclonal antibodies.

Features and Benefits

Designed to support the rapid growth of cells to high densities and the maintenance of viable cells for extended culture periods. This formulation reduces raw material cost and the low protein level reduces cost associated with downstream processing. Purified components in the product reduce the variability sometimes encountered with biological materials leading to improved consistency of the medium performance. Cells can be successfully transferred from serum-supplemented cultures to this medium with little or no adaptation.

Other Notes

This proprietary formulation includes inorganic salts, essential and non-essential amino acids, vitamins, sodium bicarbonate, HEPES, trace elements, fatty acids, and other organics. It contains low concentrations of recombinant human insulin, bovine serum albumin and human transferrin (source tested negative for HIV antibody and HbsAG). The medium does not contain L-glutamine, antibiotics, antimycotics, and phenol red.

Caution

Do not freeze. Protect from light. Several components of the medium are light labile and should not be exposed to light for lengthy periods of time. When stored at 2-8°C in the dark, medium that has not been supplemented is stable until the expiration date on the label.

Reconstitution

Prior to use, medium should be warmed to 37°C and supplemented with 50 ml/L of 200 mM L-glutamine.

Legal Information

EX-CELL is a registered trademark of Sigma-Aldrich Co. LLC

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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25G
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