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HPA005552

Sigma-Aldrich

Anti-ACSL4 antibody produced in rabbit

Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution

Synonym(s):

Anti-LACS 4 antibody produced in rabbit, Anti-Long-chain acyl-CoA synthetase 4 antibody produced in rabbit, Anti-Long-chain-acyl-CoA synthetase 4 antibody produced in rabbit, Anti-Long-chain-fatty-acid–CoA ligase 4 antibody produced in rabbit

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About This Item

UNSPSC Code:
12352203
Human Protein Atlas Number:
NACRES:
NA.43

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

product line

Prestige Antibodies® Powered by Atlas Antibodies

form

buffered aqueous glycerol solution

species reactivity

human

technique(s)

immunoblotting: 0.04-0.4 μg/mL
immunohistochemistry: 1:50-1:200

immunogen sequence

MNYLEVNRRVNNFGSGLTALGLKPKNTIAIFCETRAEWMIAAQTCFKYNFPLVTLYATLGKEAVVHGLNESEASYLITSVELLESKLKTALLDISCVKHIIYVDNKAINKAEYPEGFEIHSMQSVEELGSNPENLGIPPSRPTPSDMAIV

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... ACSL4(2182)

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General description

ACSL4 (acyl-CoA synthetase long-chain family member 4) belongs to the family of mammalian long chain acyl-CoA synthetases. This family consists of five isoforms, one being ACSL4. It is localized to the endoplasmic reticulum (ER) associated with mitochondria, and peroxisome. It is expressed in smooth muscle and neuronal cells. However, its predominant expression is in steroidogenic tissues. It is highly expressed in spleen, placenta, testis, ovary, brain and adrenal cortex. ACSL4 gene is localized to human chromosome Xq23. The encoded protein has a molecular weight of 75kDa.

Immunogen

Long-chain-fatty-acid–CoA ligase 4 recombinant protein epitope signature tag (PrEST)

Application

All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project and as a result, are supported by the most extensive characterization in the industry.

The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. These images and the collection of this vast data set can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. We also provide Prestige Antibodies® protocols and other useful information.

Biochem/physiol Actions

ACSL4 (acyl-CoA synthetase long-chain family member 4) has a higher affinity for arachidonic acid (AA) and eicosapentanoic acid (EPA), as opposed to other isoforms. Thus, it plays an essential role in AA metabolism. It is responsible for the de novo synthesis of diacylglycerol (DAG). ACSL4 also mediates the storage of fatty acids in the form of triacylglycerol. Two point mutations in this gene are linked with nonspecific mental retardation. ACSL4 expression is up-regulated in liver, colon and aggressive forms of breast cancer. It has potential as a marker for aggressive phenotype of breast cancer. This gene is linked to metabolic syndrome, as a mutation in ACSL4 gene leads to abnormal fatty acid composition of phosphatidylcholines present in plasma membrane. Inactivation of this gene is found in elliptocytosis, Alport syndrome, and mental retardation. Therefore, it is suggested that FACL4 regulates intellectual capability and coordination skills, through FACL4-derived arachidonoyl phospholipids.

Features and Benefits

Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section.

Every Prestige Antibody is tested in the following ways:
  • IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues.
  • Protein array of 364 human recombinant protein fragments.

Linkage

Corresponding Antigen APREST74278

Physical form

Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide

Legal Information

Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Lydie Cheval et al.
Function (Oxford, England), 5(4) (2024-07-10)
A detailed knowledge of the lipid composition of components of nephrons is crucial for understanding physiological processes and the development of kidney diseases. However, the lipidomic composition of kidney tubular segments is unknown. We manually isolated the proximal convoluted tubule
Ilaria Meloni et al.
Nature genetics, 30(4), 436-440 (2002-03-13)
X-linked mental retardation (XLMR) is an inherited condition that causes failure to develop cognitive abilities, owing to mutations in a gene on the X chromosome. The latest XLMR update lists up to 136 conditions leading to 'syndromic', or 'specific', mental
Xinyu Wu et al.
PloS one, 8(10), e77060-e77060 (2013-10-25)
The purpose of this study was to determine the role of long-chain fatty acyl-CoA synthetase 4 (ACSL4) in breast cancer. Public databases were utilized to analyze the relationship between ACSL4 mRNA expression and the presence of steroid hormone and human
Y Cao et al.
FEBS letters, 467(2-3), 263-267 (2000-02-17)
Fatty acid utilization is initiated by fatty acid-CoA ligase, which converts free fatty acids into fatty acyl-CoA esters. We have cloned previously the human long-chain fatty acid-CoA ligase 4 (FACL4), which is a central enzyme in controlling the free arachidonic
Miroslav Zeman et al.
The Tohoku journal of experimental medicine, 217(4), 287-293 (2009-04-07)
The composition of polyunsaturated fatty acids (PUFAs) in cell membranes and body tissues is altered in metabolic syndrome (MetS) and depressive disorder (DD). Within the cell, fatty acid coenzyme A (CoA) ligases (FACLs) activate PUFAs by esterifying with CoA. The

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