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HPA021578

Sigma-Aldrich

Anti-TRIM65 antibody produced in rabbit

Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution, Ab2

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Synonym(s):
TRIM65 Antibody - Anti-TRIM65 antibody produced in rabbit, Trim65 Antibody, Anti-Tripartite motif-containing protein 65
Human Protein Atlas Number:

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

product line

Prestige Antibodies® Powered by Atlas Antibodies

form

buffered aqueous glycerol solution

species reactivity

human

packaging

antibody small pack of 25 μL

technique(s)

immunoblotting: 0.04-0.4 μg/mL
immunofluorescence: 0.25-2 μg/mL

immunogen sequence

LELFCRTEGRCVCSVCTVRECRLHERALLDAERLKREAQLRASLEVTQQQATQAEGQLLELRKQSSQIQNSACILASWVSGKFSSLLQALEIQHTTALRS

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... TRIM65(201292)

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This Item
HPA064820SAB2103161HPA030266
conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal

product line

Prestige Antibodies® Powered by Atlas Antibodies

product line

Prestige Antibodies® Powered by Atlas Antibodies

product line

-

product line

Prestige Antibodies® Powered by Atlas Antibodies

form

buffered aqueous glycerol solution

form

buffered aqueous glycerol solution

form

buffered aqueous solution

form

buffered aqueous glycerol solution

General description

Tripartite motif 65 (TRIM65) belongs to TRIM family proteins. TRIM family contains B-box 1 and a B-box 2 type domain. The coiled domain possesses hyper-helical regions. TRIM65 exhibits a SPRY (spla and ryanodine) domain at its C-terminus. Two anti-parallel β-sheets in the SPRY domain form a curved β-sandwich. Trim65 homologous genes have been found in mammals.

Immunogen

Tripartite motif-containing protein 65 recombinant protein epitope signature tag (PrEST)

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Biochem/physiol Actions

Ubiquitination of TNRC6 proteins is through E3 ligase activity of TRIM65. The coiled coil domain plays a role in protein-protein interactions and oligomerization. It is associated with repression of miRNA activity. TRIM65 colludes and adjoins with trinucleotide repeat TNRC6 (trinucleotide repeat-containing gene 6) proteins. Genomic studies on cerebral white matter lesions have found a new locus on chromosome 17q25 accommodating TRIM65. The studies also determine a probable mechanism leading to these lesions. Uncontrolled TRIM65 activities extirpate TNRC6 stability and alter RISC (RNA-induced silencing complex) stability, miRNA activity and cellular homeostasis. Gene silencing of TRIM65 with that of siRNA leads to attenuation of let-7a reporter activity. An abnormal expression of TRIM65 has a similar effect.

Features and Benefits

Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section.

Every Prestige Antibody is tested in the following ways:
  • IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues.
  • Protein array of 364 human recombinant protein fragments.

Linkage

Corresponding Antigen APREST75679.

Physical form

Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide

Legal Information

Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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TRIM65 regulates microRNA activity by ubiquitination of TNRC6.
Li S
Proceedings of the National Academy of Sciences of the USA, 111(19), 6970-6975 (2014)
Trim65: a cofactor for regulation of the microRNA pathway.Li
Li s
RNA Biology, 11(9), 1113-1121 (2014)
TRIMmunity: the roles of the TRIM E3-ubiquitin ligase family in innate antiviral immunity.
Rajsbaum R
Journal of Molecular Biology, 426(6), 1265-1284 (2014)
TRIM family proteins and their emerging roles in innate immunity.
Ozato K
Nature Reviews: Immunology, 8(11), 849-860 (2008)
Emanuela Marchese et al.
International journal of molecular sciences, 23(16) (2022-08-27)
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