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L4144

Sigma-Aldrich

Lectin from Phaseolus vulgaris (red kidney bean)

Leucoagglutinin PHA-L, lyophilized powder, BioReagent, suitable for cell culture

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Synonym(s):
Phaseolus vulgaris agglutinin, PHA
EC Number:
NACRES:
NA.32

Quality Level

product line

BioReagent

form

lyophilized powder

potency

erythroagglutination activity (Does not agglutinate a 2% suspension of human erythrocytes at 250 μg lectin/ml.)
≤1.0 μg per mL leucoagglutination activity
≤5 μg per mL mitogenic activity (Bromdeoxyuridine incorporation in human peripheral blood mononuclear cells)

technique(s)

cell culture | mammalian: suitable

impurities

salt, free

solubility

DPBS: 5 mg/mL, slightly hazy, colorless

suitability

leucocyte agglutination activity tested

storage temp.

2-8°C

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This Item
L8902L1668L2769
form

lyophilized powder

form

lyophilized powder

form

lyophilized powder (contains ~50% buffer salts as sodium phosphate and NaCl)

form

lyophilized powder

suitability

leucocyte agglutination activity tested

suitability

-

suitability

-

suitability

-

impurities

salt, free

impurities

salt, free

impurities

-

impurities

salt, free

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

solubility

DPBS: 5 mg/mL, slightly hazy, colorless

solubility

PBS: 2 mg/mL

solubility

PBS: 5 mg/mL, clear to slightly hazy, colorless to faintly yellow (pH 6.8)

solubility

-

General description

Lectins are carbohydrate-binding proteins, which contains four subunits with a molecular weight of 125 kDa. Leucoagglutinin PHA-L is a plant lectin, which is transported by the neurons in the central nervous system.

Application

Lectin from Phaseolus vulgaris (red kidney bean) has been used:
  • it has been used as a positive control to treat cells
  • to stimulate cell culture for the analysis of lymphocyte proliferation
  • to stimulate T cells

Biochem/physiol Actions

Lectin agglutinates cells and precipitates polysaccharides and glycoproteins. It is associated with toxic allergens and hemagglutinins. Lectin exhibits anti-cancer, anti-human immunodeficiency virus (anti-HIV) and anti-microbial infection properties. Lectin inhibits mucosal atrophy, lowers type 2 diabetes and obesity, enhances nutrient absorption and regulates drug targeting.
PHA consists of two molecular species, an erythroagglutinin (PHA-E) which has low mitogenic activity and high erythroagglutinin activity, and leucoagglutinin (PHA-L) which has high mitogenic and leucoagglutinating activity, but very low erythroagglutinating activity.
PHA-E is not blood group specific, but agglutination can be inhibited by certain oligosaccharides. PHA-P is the protein form of PHA prior to separation and purification of erythroagglutinin and leucoagglutinin. PHA-M is the mucoprotein form. Conjugates are prepared from the corresponding purified lectins.

Analysis Note

Agglutination activity is expressed in μg/ml and is determined from serial dilutions of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a suspension of either human erythrocytes (2% in phosphate buffered saline, pH 6.8) or human leukocytes (107 per mL in saline) after 1 hr incubation at 25 °C.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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