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L8146

Sigma-Aldrich

Lectin from Ulex europaeus (gorse, furze)

peroxidase conjugate, lyophilized powder

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Synonym(s):
Ulex europaeus agglutinin, UEA-I
NACRES:
NA.32

biological source

Ulex europaeus

Quality Level

conjugate

peroxidase conjugate

assay

70.00-90.00% (Lowry)

form

lyophilized powder

potency

<40 μg/mL agglutination activity

peroxidase activity

≥15 units/mg protein

composition

Protein, ~80% Lowry

technique(s)

western blot: suitable

storage temp.

2-8°C

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This Item
L8262L9006L7759
technique(s)

western blot: suitable

technique(s)

-

technique(s)

-

technique(s)

western blot: suitable

biological source

Ulex europaeus

biological source

-

biological source

-

biological source

Arachis hypogaea (peanut)

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

conjugate

peroxidase conjugate

conjugate

biotin conjugate

conjugate

FITC conjugate

conjugate

peroxidase conjugate

Application

General Western Blot Protocol:
  • Glycoprotein sample size: 500ng
  • Lectin Concentration: 0.1ug/ml

  1. Load samples at 500 ng of glycoprotein per lane
  2. Run 4-20% Bis-Tris SDS page gel
  3. Transfer gel to a PVDF membrane
  4. Block membrane for 1 hr at RT with RIPA buffer (R0278 Sigma)
  5. Incubate HRP lectin at 0.1ug/ml with RIPA buffer for 2 hours at RT
  6. Wash membrane 5 x 5 minutes with 25ml RIPA buffer
  7. Detect using chemiluminescent substrate (CPS1-120)

Biochem/physiol Actions

Ulex europaeus agglutinin has anti-H blood group specificity. There are two types of lectin: UEA I has an affinity for L-fucose and UEA II has an affinity for N,N′-diacetylchitobiose. The mol. wt. of UEA I was initially found to be 170,000 daltons. Later reports indicate that UEA I may form aggregates at neutral and basic pH and that the correct mol. wt. is 68,000 daltons.

Packaging

Package size based on protein content.

Linkage

Conjugates are prepared from affinity purified lectin.

Unit Definition

One unit will form 1.0 mg purpurogallin in 20 sec from pyrogallol at pH 6.0 at 20 °C.

Physical form

Contains sodium citrate buffer salts

Preparation Note

Prepared from peroxidase, Type VI (P8375), using the two-step glutaraldehyde method of Avrameas and Ternyck.
Repurified by affinity chromatography after conjugation.

Analysis Note

Agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a 2% suspension of human blood group O erythrocytes after 1 hour incubation at 25 °C.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Xiaohu Fan et al.
Human gene therapy, 21(7), 877-890 (2010-02-19)
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The IκB kinase (IKK)-NF-κB signaling pathway plays a multifaceted role in inflammatory bowel disease (IBD): on the one hand, it protects from apoptosis; on the other, it activates transcription of numerous inflammatory cytokines and chemokines. Although several murine models of
F Bucardo et al.
Zoonoses and public health, 63(8), 600-607 (2016-05-14)
Information about porcine norovirus (PoNoV), genetically similar to human NoV (HuNoV), is limited from rural areas where household-raised pigs are heavily exposed to faecal material which could facilitate transmission. Histo-blood group antigens (HBGAs) are known susceptibility factors to NoV in
K Nozaki et al.
Journal of neurology, neurosurgery, and psychiatry, 80(8), 904-908 (2009-02-26)
To study the clinical and pathological correlations of neuromuscular patients with a high aldolase and normal creatine kinase (CK) in serum at presentation or during a symptomatic exacerbation. Records and muscle biopsies were retrospectively reviewed in a consecutive series of
Peroxidase labelled antibody and Fab conjugates with enhanced intracellular penetration.
S Avrameas et al.
Immunochemistry, 8(12), 1175-1179 (1971-12-01)

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