MilliporeSigma
All Photos(1)

Documents

L9234

Sigma-Aldrich

LB Broth (Lennox)

Liquid microbial growth medium

Sign Into View Organizational & Contract Pricing

grade

for molecular biology

sterility

sterile; 0.2 μm filtered

form

liquid

suitability

suitable for nonselective for Escherichia coli and Coliforms

storage temp.

room temp

General description

Liquid media is also termed as broth. It has nutrients dissolved in water. Luria-Bertani broth (LB broth) is used in naphthalene degradation experiments.

Application

LB Broth has been used to culture P. aeruginosa.
Suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added.

Biochem/physiol Actions

LB Lennox media is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates.

Features and Benefits

LB Lennox media provides:
  • Filter-sterilized, ready-to-use format
  • Convenient package size
  • Standard formulation

Components

10 g/L Tryptone
5.0 g/L Yeast Extract
5.0 g/L NaCl

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Customers Also Viewed

Slide 1 of 7

1 of 7

Pseudomonas toxin pyocyanin triggers autophagy: Implications for pathoadaptive mutations
Yang ZS, et al.
Autophagy, 12(6), 1015-1028 (2016)
Understanding Microbes: A Laboratory Textbook for Microbiology (1989)
Su-Yun Chang et al.
Water environment research : a research publication of the Water Environment Federation, 87(1), 61-67 (2015-01-30)
Luria-Bertani broth and acetone were usually used in naphthalene degradation experiments as nutrient and solvent. However, their effect on the degradation was seldom mentioned. In this work, we investigated the effect of LB, naphthalene concentration, and acetone on the degradation

Protocols

Initiating a Starter Culture

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service