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M2441

Sigma-Aldrich

Anti-MAGI-2 antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

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Synonym(s):
Anti-ARIP, Anti-S-SCAM
MDL number:
NACRES:
NA.46

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

rat

technique(s)

microarray: suitable
western blot: 1:250 using rat brain extracts

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

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This Item
M5691M2694M4937
biological source

rabbit

biological source

rabbit

biological source

rabbit

biological source

rabbit

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

technique(s)

microarray: suitable, western blot: 1:250 using rat brain extracts

technique(s)

microarray: suitable, western blot: 1:500 using rat brain extracts

technique(s)

indirect immunofluorescence: 1:250-1:500 using mouse fibroblast NIH3T3 cell line, microarray: suitable, western blot: 1:2,000-1:4,000 using rat brain extract (S1 fraction) and PC12 whole cell extract.

technique(s)

microarray: suitable, western blot: 1:2,000 using rat brain homogenate extract

species reactivity

rat

species reactivity

rat

species reactivity

mouse, rat

species reactivity

rat, mouse (predicted)

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

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General description

MAGI-2 is composed of three domains including six PDZ, one guanylate kinase (GK) and two WW domain.
The MAGUK (Membrane Associated Guanylate kinase) family of proteins localize to regions of cell-cell contact, such as tight junctions in epithelial cells and synaptic junctions in neurons, and are believed to be involved in the assembly of multiprotein complexes via their protein-protein interaction domains.2

MAGI-2 was initially identified in rat, as a protein interacting with N-methyl-D-aspartate receptors (NMDA-R) and neuronal cell adhesion proteins, and was named S-SCAM.4Three isoforms of S-SCAM were identified, of 1277, 1113 and 1053 amino acids length, respectively.5

Immunogen

synthetic peptide corresponding to amino acids 554-571 of MAGI-2/S-SCAM conjugated to KLH.

Application

Anti-MAGI-2 antibody produced in rabbit has been used in immunoblotting and immunolabeling.

Biochem/physiol Actions

Membrane associated guanylate kinase inverted-2 (MAGI-2) as well as MAGI-3, was shown to interact with the tumor suppressor PTEN through one of its PDZ domains, apparently acting as a scaffolding protein that could assemble a multi subunit signaling complex. It is known to be involved in the assembly of multiprotein complexes via their protein-protein interaction domains.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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Stargazin and other transmembrane AMPA receptor regulating proteins interact with synaptic scaffolding protein MAGI-2 in brain
Deng F, et al.
The Journal of Neuroscience, 26(30), 7875-7884 (2006)
S-SCAM/MAGI-2 is an essential synaptic scaffolding molecule for the GluA2-containing maintenance pool of AMPA receptors
Danielson E, et al.
The Journal of Neuroscience, 32(20), 6967-6980 (2012)
SynArfGEF is a guanine nucleotide exchange factor for Arf6 and localizes preferentially at post-synaptic specializations of inhibitory synapses
Fukaya M, et al.
Journal of Neurochemistry, 116(6), 1122-1137 (2011)
Synaptic localization and function of Sidekick recognition molecules require MAGI scaffolding proteins
Yamagata M and Sanes JR
The Journal of Neuroscience, 30(10), 3579-3588 (2010)
Bingbing Zhu et al.
Kidney international, 96(3), 642-655 (2019-06-07)
The essential role of membrane associated guanylate kinase 2 (MAGI2) in podocytes is indicated by the phenotypes of severe glomerulosclerosis of both MAGI2 knockout mice and in patients with congenital nephrotic syndrome (CNS) caused by mutations in MAGI2. Here, we

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