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M3795

Sigma-Aldrich

IgM, Kappa from murine myeloma

clone TEPC 183, purified immunoglobulin, buffered aqueous solution

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Synonym(s):
Mouse IgM-κ
MDL number:
NACRES:
NA.46

biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

clone

TEPC 183, monoclonal

Assay

≥90% (HPLC)

form

buffered aqueous solution

concentration

2.0-2.2 mg/mL (Determined by the absorbance at 280 nm (E1%/280 = 11.8). Each vial contains at least 1 mg of purified myeloma protein.)

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

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1 of 4

This Item
M1520M1421M1398
conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody form

ascites fluid

antibody form

purified immunoglobulin

antibody form

ascites fluid

clone

TEPC 183, monoclonal

clone

TEPC 183, monoclonal

clone

TEPC 15, monoclonal

clone

MOPC 31C, monoclonal

form

buffered aqueous solution

form

lyophilized powder

form

buffered aqueous solution

form

lyophilized powder

concentration

2.0-2.2 mg/mL (Determined by the absorbance at 280 nm (E1%/280 = 11.8). Each vial contains at least 1 mg of purified myeloma protein.)

concentration

-

concentration

-

concentration

-

General description

IgM antibodies are present as pentamers in the serum and are produced in response to antigens IgM, κ from murine myeloma is produced by TEPC 183 tumor line introduced subcutaneously in BALB/c mice.
IgM is a highly conserved antibody in vertebrates and is expressed early during immune response. It is secreted by peritoneal B cells.

Specificity

The TEPC 183 tumor line is pristine-induced plasmacytoma-originated and carried subcutaneously in BALB/c mice. The hapten binding specificity of the TEPC 183 line has not been determined. It specifically recognizes mouse IgM. Identity and purity of the immunoglobulin is established by immunoelectrophoresis.

Application

IgM, Kappa from murine myeloma has been used in:
  • sandwich enzyme linked immunosorbent assay (ELISA)
  • flow cytometry
  • dot-immunobinding assay

Biochem/physiol Actions

IgM plays a key role in the engulfing of apoptotic cells. A reduction in serum IgM levels leads to increased autoimmune response and higher risk for infections. IgM displays polyreactive and autoreactive functionality. It plays a key role in tissue homeostasis by mediating clearance of tissue based molecules. IgM has sites for N-linked glycosylation, with sugars namely, mannose, galactose, N-acetyl glucosamine and sialic acid. IgM linked agarose resins have been tested for efficient conjugate binding.

Physical form

Solution in 0.05 M Tris, 0.5 M sodium chloride, pH 8.0, containing 0.02% sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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