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M7194

Sigma-Aldrich

Monoclonal Anti-MEKK4 antibody produced in mouse

~2 mg/mL, clone MEKK4-338, purified immunoglobulin, buffered aqueous solution

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Synonym(s):
Anti-MAP3K4
MDL number:
NACRES:
NA.44

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

MEKK4-338, monoclonal

form

buffered aqueous solution

mol wt

antigen ~182 kDa

species reactivity

human, rat, mouse

concentration

~2 mg/mL

technique(s)

immunocytochemistry: suitable
indirect ELISA: suitable
microarray: suitable
western blot: 10-20 μg/mL using total cell extract of HEK293T cells

isotype

IgG2b

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... MAP3K4(4216)
mouse ... Map3k4(26407)
rat ... Map3k4(308106)

General description

Monoclonal Anti-MEKK4 (mouse IgG2b isotype) is derived from the hybridoma MEKK4-338 produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with a synthetic peptide of human MEKK4. MAP kinase 4 (MEKK4 also called MAP3K4, is the human homolog of the S. cerevisiae Ssk2 and Ssk22 MAPKKKs. The protein has 1607 amino acids with a protein kinase catalytic domain at the C-terminus that is 98, 42, 36 and 41 percent identical to the kinase domains of mouse MEKK4, Serine/threonine-protein kinase proteins (SSK22 - Ssk2, Ssk22) and S. pombe Wik1, respectively. A non-kinase domain that has a regulatory function is located at the N-terminal part of the protein. The protein is expressed mainly in heart, placenta, skeletal muscle and pancreas.

Specificity

Monoclonal Anti-MEKK4 antibody is specific for human, rat and mouse MEKK4 (approx. 182 kDa).

Immunogen

synthetic peptide corresponding to amino acids 1081-1095 of human MEKK4, conjugated to KLH.

Application

Monoclonal Anti-MEKK4 antibody produced in mouse has been used in:
  • western blotting
  • ELISA
  • immunocytochemistry

Biochem/physiol Actions

MAP kinase 4 (MEKK4) -null embryos developed highly penetrant neural tube defects with exencephaly and spina bifida. During neural tube development, it regulates MAP kinase 4 (MKK4) activity and apoptotic cell death.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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