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MAK037

Sigma-Aldrich

NAD/NADH Quantitation Kit

sufficient for 100 colorimetric tests

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Synonym(s):
NAD/NADH Assay Kit
eCl@ss:
32161000

usage

sufficient for 100 colorimetric tests

detection method

colorimetric

relevant disease(s)

cancer

storage temp.

−20°C

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NAD/NADH Quantitation Kit sufficient for 100 colorimetric tests

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NAD+/NADH Assay Kit Sufficient for 100 fluorometric tests

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detection method

colorimetric

detection method

colorimetric, fluorometric

detection method

colorimetric, fluorometric

detection method

fluorometric

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

relevant disease(s)

cancer

relevant disease(s)

cardiovascular diseases

relevant disease(s)

cancer; cardiovascular diseases

relevant disease(s)

cancer, Alzheimer′s disease; neurological disorders, Parkinson′s disease; aging/geriatric diseases

General description

Nicotinamide adenine dinucleotide (NAD) is an enzymatic cofactor involved in many redox reactions. NAD functions as an electron carrier, cycling between the oxidized (NAD) and reduced (NADH) forms. In addition to its role in redox reactions, NAD plays critical roles in ADP (adenosine diphosphate)-ribosylation reactions and as a substrate for sirtuins.

Application

NAD/NADH Quantitation Kit has been used to quantify NAD+/NADH influenced by SIRT1 (sirtuin 1) activity (associated with ischemia-reperfusion injury) in the presence of trimetazidine.

Suitability

Suitable for the detection of NADH and NAD in animal tissues (liver, kidney etc.), cell culture (adherent or suspension cells), serum or urine.

Principle

The NAD/NADH Quantification Kit provides a convenient tool for sensitive detection of the intracellular nucleotides, NAD (nicotinamide adenine dinucleotide) and NADH, and their ratio without the requirement to purify NAD/NADH from samples. This assay is specific for NAD and NADH, and does not detect NADP or NADPH. NADt (NAD and NADH) or NADH are quantified in a colorimetric assay (450 nm).

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signalword

Warning

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3


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