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MAK041

Sigma-Aldrich

β-Hydroxybutyrate Assay Kit

sufficient for 100 colorimetric tests

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NACRES:
NA.84

usage

sufficient for 100 colorimetric tests

detection method

colorimetric

relevant disease(s)

endocrinological disorders, diabetes; cancer; gastrointestinal diseases

storage temp.

−20°C

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MAK041

β-Hydroxybutyrate Assay Kit

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MAK016

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MAK126

Bilirubin Assay Kit

detection method

colorimetric

detection method

colorimetric, fluorometric

detection method

colorimetric

detection method

colorimetric

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

relevant disease(s)

endocrinological disorders, diabetes; cancer; gastrointestinal diseases

relevant disease(s)

-

relevant disease(s)

hematological disorder; gastrointestinal diseases

relevant disease(s)

endocrinological disorders, diabetes; cancer

General description

β-Hydroxybutyrate, also known as 3-Hydroxy-butyrate, is a ketone body primarily synthesized in the liver from the oxidation of fatty acids. Ketone body formation, ketogenesis, is elevated when blood glucose levels drop. Moderately elevated blood ketone bodies occur normally during fasting and prolonged exercise. During alcoholic or diabetic ketoacidosis, blood levels of β-hydroxybutyrate can rise to abnormally high levels. β-hydroxybutyrate can be used as a marker for hepatic energy metabolism. β-hydroxybutyrate formation increases with excessive acetyl-CoA, and serves as a source of energy to the brain.

Application

β-Hydroxybutyrate Assay Kit has been used
  • to quantify the level of β-hydroxybutyrate in plasma/serum
  • to study the change in myocardial 18F-FDG uptake upon extended ketogenic diet, by measuring the level of plasma ketone bodies
  • to study the role of β-hydroxybutyrate in the treatment of impairment and neuropathic pain in spinal cord-injured mice

Suitability

Suitable for the measurement β-Hydroxybutyrate levels in biological samples including serum and urine

Principle

In this assay, β-Hydroxybutyrate concentration is determined by a coupled enzyme reaction, which results in a colorimetric (450 nm) product, proportional to the β-Hydroxybutyrate present.

pictograms

Corrosion

signalword

Danger

hcodes

Hazard Classifications

Eye Dam. 1 - Skin Corr. 1B

Storage Class

8A - Combustible, corrosive hazardous materials

wgk_germany

WGK 3


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Yongsheng Yu et al.
European journal of pharmacology, 829, 121-128 (2018-04-22)
The present study was designed to examine the protection of D-β-hydroxybutyrate (BHB) against ischemia/reperfusion (I/R) injury in heart and investigate its underlying mechanism. Male adult mice were exposed to 30 min of ischemia and 24 h of reperfusion. Osmotic pumps were implanted
Ning Zhang et al.
Food & function, 11(6), 5647-5663 (2020-06-17)
Ketogenic diet (KD) is defined as a high-fat, low-carbohydrate diet with appropriate amounts of protein, which has broad neuroprotective effects. However, the mechanisms of ameliorating the demyelination and of the neuroprotective effects of KD have not yet been completely elucidated.
Tumor metabolism, the ketogenic diet and β-hydroxybutyrate: novel approaches to adjuvant brain tumor therapy
Woolf E C, et al.
Frontiers in Molecular Neuroscience, 9, 122-122 (2016)
Skeletal muscle IL-6 and regulation of liver metabolism during high-fat diet and exercise training.
Knudsen J G, et al.
Physiological Reports, 4(9), e12788-e12788 (2016)
Jenni Määttä et al.
Scientific reports, 8(1), 13583-13583 (2018-09-13)
Hypoxia of residence at high altitude (>2500 m) decreases birth weight. Lower birth weight associates with infant mortality and morbidity and increased susceptibility to later-in-life cardiovascular and metabolic diseases. We sought to determine the effects of hypoxia on maternal glucose and

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