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MAK044

Sigma-Aldrich

Free Fatty Acid Quantitation Kit

sufficient for 100 colorimetric or fluorometric tests

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Synonym(s):
Free Fatty Acid Assay Kit

usage

sufficient for 100 colorimetric or fluorometric tests

application(s)

cosmetics
food and beverages

detection method

colorimetric
fluorometric

relevant disease(s)

endocrinological disorders, diabetes; cancer

storage temp.

−20°C

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detection method

colorimetric, fluorometric

detection method

fluorometric

detection method

colorimetric, fluorometric

detection method

colorimetric, fluorometric

usage

sufficient for 100 colorimetric or fluorometric tests

usage

sufficient for 100 fluorometric tests

usage

sufficient for 100 colorimetric or fluorometric tests

usage

sufficient for 100 colorimetric or fluorometric tests

application(s)

cosmetics
food and beverages

application(s)

-

application(s)

-

application(s)

-

relevant disease(s)

endocrinological disorders, diabetes; cancer

relevant disease(s)

endocrinological disorders, diabetes; gastrointestinal diseases; cancer

relevant disease(s)

endocrinological disorders, diabetes; cardiovascular diseases; cancer

relevant disease(s)

-

General description

Free fatty acids, also know as nonesterified fatty acids, circulate in the plasma bound to albumin. The majority of free fatty acids are derived from either dietary sources or are mobilized from adipose tissue. Plasma free fatty acids are elevated in many obesity-related disorders and may contribute to insulin resistance in peripheral tissues. Conditions such as sepsis and tumors producing lipoactive homones may also be associated with elevated free fatty acid levels.

Abnormalities in fatty acid metabolism causes fatty liver disease. Accumulation of free fatty acid in myocytes leads to the risk of cardiac dysfunction. Increased fatty acid β-oxidation is implicated in cardiac ischemia.

Application

Free Fatty Acid Quantitation Kit has been used in the following:
  • to measure lipid hydrolysis, by determining the free fatty acid in digested sample.
  • to measure plasma and serum free fatty acids level.
  • to quantify free fatty acids level in liver tissue.

Suitability

Suitable for detecting the long-chain free fatty acids in various biological samples, such as serum, plasma and other body fluids, food, growth media, etc.

Principle

In this kit, the concentration of fatty acids (C8 and longer) is determined by a coupled enzyme assay, which results in a colorimetric (570 nm)/ fluorometric (λex = 535/λem = 587 nm) product, proportional to the fatty acids present.

Related product

Product No.
Description
Pricing

replaced by

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

188.6 °F - closed cup

flash_point_c

87 °C - closed cup


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