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MAK045

Sigma-Aldrich

HDL and LDL/VLDL Quantitation Kit

sufficient for 100 colorimetric or fluorometric tests

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Synonym(s):
HDL and LDL/VLDL Assay Kit
NACRES:
NA.25

usage

sufficient for 100 colorimetric or fluorometric tests

application(s)

cosmetics
food and beverages

detection method

colorimetric
fluorometric

relevant disease(s)

cardiovascular diseases

storage temp.

−20°C

General description

Lipoproteins transport the majority of plasma lipids including cholesterol and triglycerides. High-density lipoprotein (HDL), low-density lipoprotein (LDL), and very-low-density lipoprotein (VLDL) are the lipoproteins responsible for the vast majority of cholesterol transport in the blood. High LDL levels and low HDL levels are strongly associated with increased risk of adverse cardiovascular events.

Application

HDL and LDL/VLDL Quantitation Kit has been used to determine the concentrations of HDL and LDL/VLDL in serum samples.

Suitability

Suitable for use with plasma and serum samples.

Principle

In this kit, serum HDL and LDL/VLDL are first separated and then the cholesterol concentration of each is determined by a coupled enzyme assay, which results in a colorimetric (570 nm)/fluorometric (λex = 535/λem = 587 nm) product, proportional to the cholesterol present. This kit can also be used to determine the concentration of free cholesterol and cholesteryl esters present in a sample.

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

188.6 °F - closed cup

flash_point_c

87 °C - closed cup


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