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MAK064

Sigma-Aldrich

Lactate Assay Kit

sufficient for 100 colorimetric or fluorometric tests

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Synonym(s):
Lactic Acid Assay Kit
NACRES:
NA.84

usage

sufficient for 100 colorimetric or fluorometric tests

application(s)

cosmetics
food and beverages

detection method

colorimetric
fluorometric

relevant disease(s)

endocrinological disorders, diabetes; cancer

storage temp.

−20°C

General description

L(+)-Lactate is a metabolic compound formed in animals by the action of the enzyme Lactate Dehydrogenase. Lactate is produced in proliferating cells and during anaerobic conditions such as exercise. Abnormally high concentrations of lactate have been related to pathological conditions such as cancer, diabetes, and lactate acidosis. L(+)-Lactate is the major stereoisomer of lactate formed in human intermediary metabolism and is present in blood at levels of around 1-2 mmol/L. This kit provides a convenient means for detecting L(+)-Lactate in biological samples. There is no need of pretreatment or purification of samples.

Application

Lactate Assay Kit has been used to determine the concentrations of lactate in samples.

Suitability

Suitable for detecting L(+)-Lactate in culture medium, fermentation medium, blood and cells.

Principle

In this assay, lactate concentration is determined by an enzymatic assay, which results in a colorimetric (570 nm)/ fluorometric (λex = 535 nm/λem = 587 nm) product, proportional to the lactate present. Typical levels of sensitivity for this kit are between 0.001-10 mM.

pictograms

Health hazard

signalword

Danger

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

188.6 °F - closed cup

flash_point_c

87 °C - closed cup


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