MAK189
A-KG Dehydrogenase Activity Assay Kit
Sufficient for 100 Colorimetric tests
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Synonym(s):
α-KG Dehydrogenase Activity Assay Kit, α-Ketoglutarate Dehydrogenase Activity Colorimetric Assay Kit, AKGDH Assay Kit, Alpha-Ketoglutarate Dehydrogenase Activity Colorimetric Assay Kit, OGDC Activity Assay Kit, Oxoglutarate Dehydrogenase Assay Kit
UNSPSC Code:
12161503
NACRES:
NA.84
Recommended Products
detection method
colorimetric
relevant disease(s)
endocrinological disorders, diabetes; orthopedic diseases; neurological disorders; rheumatological diseases; cancer; cardiovascular diseases
storage temp.
−20°C
Gene Information
human ... OGDH(4967)
mouse ... OGDH(18293)
rat ... OGDH(360975)
General description
α-Ketoglutarate Dehydrogenase (α-KGDH, EC 1.2.4.2) is a highly regulated Krebs cycle enzyme, forms the E1 subunit of the mitochondrial 2-oxoglutarate dehydrogenase complex. It catalyzes the conversion of α-ketoglutarate to succinyl-CoA and produces NADH directly providing electrons for the respiratory chain. Decreased α-KGDH complex activity has been associated with oxidative stress and neurodegenerative disorders such as Alzheimer′s and Parkinson′s diseases.
Application
α-Ketoglutarate Dehydrogenase Activity Colorimetric Assay Kit has been used to measure the enzyme activity of α-ketoglutarate dehydrogenase in samples.
Suitability
Suitable for the detection of α-ketoglutarate dehydrogenase activity in samples such as tissue, cells, and purified mitochondria.
Principle
The α-Ketoglutarate Dehydrogenase Activity Colorimetric Assay Kit provides a simple procedure for measuring α-ketoglutarate dehydrogenase activity in a variety of tissues, cells, and isolated mitochondria. α-KGDH activity is determined by measuring a colorimetric product with absorbance at 450 nm (A450) proportional to the enzymatic activity present. One unit of α-ketoglutarate dehydrogenase is the amount of enzyme that generates 1.0 μmole of NADH per minute at pH 7.5 at 37 °C.
Kit Components Only
Product No.
Description
- KGDH Assay Buffer
- KGDH Substrate
- KGDH Developer
- NADH Standard
- KGDH Positive Control
signalword
Danger
hcodes
Hazard Classifications
Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1B
wgk_germany
WGK 3
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