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MAK199

Sigma-Aldrich

Acetoacetate Colorimetric Assay Kit

Sufficient for 100 Colorimetric tests

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Synonym(s):
AcAc Assay Kit
NACRES:
NA.84

detection method

colorimetric

relevant disease(s)

endocrinological disorders, diabetes; cancer

storage temp.

−20°C

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MAK082MAK102MAK068
storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

relevant disease(s)

endocrinological disorders, diabetes; cancer

relevant disease(s)

cardiovascular diseases; cancer; hematological disorder

relevant disease(s)

endocrinological disorders, diabetes; cancer

relevant disease(s)

cancer; cardiovascular diseases; hematological disorder

General description

Acetoacetate (AcAc) is a β-ketoacid formed in the mitochondria by the condensation of two molecules of acetyl-CoA. AcAc is either enzymatically reduced to 3-hydroxybutyrate or decarboxylated to produce acetone. Ketone bodies (AcAc, 3-HB, and acetone) are used as alternate source of energy in the absence of glucose. Excess ketone bodies are observed in type I diabetes, alcoholism, or starvation.

Suitability

Suitable for the detection of acetoacetate in samples such as serum, plasma, blood, urine, and other body fluids.

Principle

The Acetoacetate Colorimetric Assay kit provides a simple and sensitive procedure for measuring AcAc in various samples (ranging from 20-100 nmole/well). AcAc concentration is determined by generating a colorimetric (550 nm) product proportional to the amount of AcAc present. The kit does not detect 3-hydroxybutyrate and is specific for acetoacetate.

Kit Components Only

Product No.
Description

  • Acetoacetate Assay Buffer

  • Acetoacetate Standard

  • Acetoacetate Substrate

replaced by

Product No.
Description
Pricing

pictograms

Exclamation mark

signalword

Warning

hcodes

Hazard Classifications

Acute Tox. 4 Oral

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3


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