All Photos(2)




Acetoacetate Colorimetric Assay Kit

Sufficient for 100 Colorimetric tests

Sign Into View Organizational & Contract Pricing

AcAc Assay Kit

detection method


relevant disease(s)

endocrinological disorders, diabetes; cancer

storage temp.


Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
storage temp.


storage temp.


storage temp.


storage temp.


relevant disease(s)

endocrinological disorders, diabetes; cancer

relevant disease(s)

cardiovascular diseases; cancer; hematological disorder

relevant disease(s)

endocrinological disorders, diabetes; cancer

relevant disease(s)

cancer; cardiovascular diseases; hematological disorder

General description

Acetoacetate (AcAc) is a β-ketoacid formed in the mitochondria by the condensation of two molecules of acetyl-CoA. AcAc is either enzymatically reduced to 3-hydroxybutyrate or decarboxylated to produce acetone. Ketone bodies (AcAc, 3-HB, and acetone) are used as alternate source of energy in the absence of glucose. Excess ketone bodies are observed in type I diabetes, alcoholism, or starvation.


Suitable for the detection of acetoacetate in samples such as serum, plasma, blood, urine, and other body fluids.


The Acetoacetate Colorimetric Assay kit provides a simple and sensitive procedure for measuring AcAc in various samples (ranging from 20-100 nmole/well). AcAc concentration is determined by generating a colorimetric (550 nm) product proportional to the amount of AcAc present. The kit does not detect 3-hydroxybutyrate and is specific for acetoacetate.

Kit Components Only

Product No.

  • Acetoacetate Assay Buffer

  • Acetoacetate Standard

  • Acetoacetate Substrate

replaced by

Product No.


Exclamation mark




Hazard Classifications

Acute Tox. 4 Oral

Storage Class

10 - Combustible liquids



Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).


Product Number
Pack Size/Quantity

Additional examples:





enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Customers Also Viewed

Slide 1 of 4

1 of 4

Anna Go et al.
Sensors (Basel, Switzerland), 21(14) (2021-07-25)
In this report, we present an enzyme deposited Au electrode for an electrochemical measurement of acetylacetic acid (AcAc) in urine. The electrode has an immobilized layer of a mixture of D-β-hydroxybutyrate dehydrogenase (HBDH) and nicotinamide adenine dinucleotide (NADH) as sensing
Tae Woong Whon et al.
EMBO reports, 22(1), e50663-e50663 (2020-11-24)
Castration of young males is widely used in the cattle industry to improve meat quality, but the mechanism linking hypogonadism and host metabolism is not clear. Here, we use metataxonomic and metabolomic approaches to evaluate the intestinal microbiota and host
Daniela Liśkiewicz et al.
Frontiers in cellular neuroscience, 15, 733607-733607 (2021-08-31)
Experimental and clinical data support the neuroprotective properties of the ketogenic diet and ketone bodies, but there is still a lot to discover to comprehensively understand the underlying mechanisms. Autophagy is a key mechanism for maintaining cell homeostasis, and therefore
Enrique Chávez et al.
The Journal of pharmacology and experimental therapeutics, 361(2), 292-302 (2017-02-18)
Background: Mitochondrion is an important metabolic and energetic organelle that regulates several cellular processes. Mitochondrial dysfunction has been related to liver diseases including hepatocellular carcinoma. As a result, the energetic demand is not properly supplied and mitochondrial morphologic changes have

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service