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MAK211

Sigma-Aldrich

Lipolysis Colorimetric Assay Kit

Sufficient for 100 Colorimetric tests

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EC Number:
NACRES:
NA.84

detection method

colorimetric

relevant disease(s)

endocrinological disorders, diabetes

storage temp.

−20°C

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Lipolysis Colorimetric Assay Kit Sufficient for 100 Colorimetric tests

MAK211

Lipolysis Colorimetric Assay Kit

Adipolysis Assay Kit sufficient for 200 colorimetric or fluorometric tests

MAK313

Adipolysis Assay Kit

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

relevant disease(s)

endocrinological disorders, diabetes

relevant disease(s)

endocrinological disorders, diabetes; obesity; cardiovascular diseases; cancer

relevant disease(s)

cancer

relevant disease(s)

cancer

General description

Lipolysis is the process of hydrolyzing triglycerides to free fatty acids and glycerol. This process involves the action of adipose TG lipase (ATGL), hormone-sensitive lipase (HSL), and monoglyceride lipase. Lipolysis maintains the energy balance during fasting and exercise by providing a substrate for oxidative metabolism. Lipolysis is regulated by nutritional factors and hormones. Problems with the regulation of lipolysis is associated with obesity, diabetes, and metabolic syndromes.

Suitability

Suitable for the measurement of lipolysis in 3T3-L1 and adipocytes.

Principle

The Lipolysis (3T3-L1) Colorimetric Assay Kit contains a synthetic catecholamine (isoproterenol) that activates β-adrenergic receptors. This activates adenylate cyclase, converting ATP to cAMP. cAMP then activates the hydrolysis of triglycerides by hormone-sensitive lipase. Lipolysis is determined by measuring a colorimetric product with absorbance at 570 nm (A570) proportional to the amount of glycerol present.

Kit Components Only

Product No.
Description

  • Lipolysis Assay Buffer

  • Lipolysis Wash Buffer

  • Glycerol Assay Buffer

  • Glycerol Probe, in DMSO

  • Glycerol Enzyme Mix

  • Glycerol Standard, 100 mM

  • Isoproterenol, 10 mM

replaced by

pictograms

Health hazard

signalword

Danger

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3


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Lipolysis?a highly regulated multi-enzyme complex mediates the catabolism of cellular fat stores.
Lass A, et al.
Progress in Lipid Research, 50(1), 14-27 (2011)
Control of fatty acid and glycerol release in adipose tissue lipolysis.
Langin D.
Comptes rendus biologies, 329(8), 598-607 (2006)
John M Dean et al.
Cell reports, 33(1), 108228-108228 (2020-10-08)
The Mediator complex relays regulatory signals from gene-specific transcription factors to the basal transcriptional machinery. However, the role of individual Mediator subunits in different tissues remains unclear. Here, we demonstrate that MED19 is essential for adipogenesis and maintenance of white

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