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MAK344

Sigma-Aldrich

Cell Cycle Analysis Kit

sufficient for 100 assays

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usage

sufficient for 100 assays

detection method

fluorometric

relevant disease(s)

cancer

storage temp.

−20°C

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MAK057MAK165MAK166
Cell Cycle Analysis Kit sufficient for 100 assays

MAK344

Cell Cycle Analysis Kit

Citrate Assay Kit sufficient for 100 colorimetric or fluorometric tests

MAK057

Citrate Assay Kit

detection method

fluorometric

detection method

colorimetric, fluorometric

detection method

fluorometric

detection method

fluorometric

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

relevant disease(s)

cancer

relevant disease(s)

cancer

relevant disease(s)

cardiovascular diseases; aging/geriatric diseases; orthopedic diseases; pulmonary disorders; neurological disorders

relevant disease(s)

orthopedic diseases; cardiovascular diseases; aging/geriatric diseases; neurological disorders; pulmonary disorders

General description

Cell cycle is a ubiquitous, complex sequence of events leading to growth and proliferation of cells. Cell cycle progression is tightly regulated due to its involvement in development, DNA damage and repair, etc. Anomalies in cell cycle progression can lead to tissue hyperplasia and diseases such as cancer. Cell cycle can be subdivided into interphase (G0/G1, S and G2) and mitotic (M) phase (prophase, metaphase, anaphase and telophase).

Features and Benefits

The method is non-radioactive, rapid and accurate and can be used for high throughput cell cycle analysis with contemporary flow cytometer instruments.

Suitability

Suitable for studying cell cycle in adherent or suspension cells

Principle

The kit utilizes a nuclear dye, the binding of which to nucleic acids in the cell results in fluorescence signal, which is proportional to cellular DNA content. The percentages of cells in different phases of the cell cycle (G0/G1, S, and G2/M) can be quantified by flow cytometry. 

Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable


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